Abstract

Indonesia is the largest country of oil palm production in the world. The oil palm by-product is still containing almost 80% of carbohydrates, such as cellulose and hemicellulose. This study aimed to optimize the enzymatic degradation of oil palm leaves in order to achieve zero waste of oil palm biomass by-product. Sodium hydroxide (NaOH) solution was used to isolate hemicellulose extraction from oil palm leaves. Various concentrations of NaOH such as 2 M, 2.5 M, 3 M, 3.5 M and 4 M and the reflux times of 2 hours, 4 hours, and 6 hours were used to optimize hemicellulose extraction. The optimum condition for hemicellulose extraction was 3 M NaOH and reflux time was 6 hours, while the yield at that condition was 48.94 % (w/w). The extracted hemicellulose is then hydrolyzed by a xylanolytic enzyme from recombinant Escherichia coli DH5α (namely pTP510). Temperatures of incubation were varied 50℃, 60℃, 70℃, and 80℃ for 24 hours at pH 6. The hydrolysis result was analyzed using High Performance Liquid Chromatography (HPLC). Xylose was produced at an optimum temperature of 60℃ (120.231 ppm), arabinose at 50℃ (26.265 ppm) whereas xylo-oligosaccharide at 50℃ (280.465 ppm).

Original languageEnglish
Title of host publication1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
EditorsArika Indah Kristiana, Ridho Alfarisi
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735443006
DOIs
Publication statusPublished - 4 Jan 2023
Event1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021 - Jember, Indonesia
Duration: 18 Sept 202119 Sept 2021

Publication series

NameAIP Conference Proceedings
Volume2679
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
Country/TerritoryIndonesia
CityJember
Period18/09/2119/09/21

Fingerprint

Dive into the research topics of 'Optimalization of Enzymatic Degradation on Oil Palm Leaves Hemicellulose'. Together they form a unique fingerprint.

Cite this