TY - GEN
T1 - Optimalization of Enzymatic Degradation on Oil Palm Leaves Hemicellulose
AU - Kurniati, Anita
AU - Purwani, Ni Nyoman
AU - Laras, Galih Ayhusta
AU - Rohmawati,
AU - Rohman, Ali
AU - Baktir, Afaf
AU - Suwito, Hery
AU - Sakka, Kazuo
AU - Sakka, Makiko
AU - Puspaningsih, Ni Nyoman Tri
N1 - Publisher Copyright:
© 2023 American Institute of Physics Inc.. All rights reserved.
PY - 2023/1/4
Y1 - 2023/1/4
N2 - Indonesia is the largest country of oil palm production in the world. The oil palm by-product is still containing almost 80% of carbohydrates, such as cellulose and hemicellulose. This study aimed to optimize the enzymatic degradation of oil palm leaves in order to achieve zero waste of oil palm biomass by-product. Sodium hydroxide (NaOH) solution was used to isolate hemicellulose extraction from oil palm leaves. Various concentrations of NaOH such as 2 M, 2.5 M, 3 M, 3.5 M and 4 M and the reflux times of 2 hours, 4 hours, and 6 hours were used to optimize hemicellulose extraction. The optimum condition for hemicellulose extraction was 3 M NaOH and reflux time was 6 hours, while the yield at that condition was 48.94 % (w/w). The extracted hemicellulose is then hydrolyzed by a xylanolytic enzyme from recombinant Escherichia coli DH5α (namely pTP510). Temperatures of incubation were varied 50℃, 60℃, 70℃, and 80℃ for 24 hours at pH 6. The hydrolysis result was analyzed using High Performance Liquid Chromatography (HPLC). Xylose was produced at an optimum temperature of 60℃ (120.231 ppm), arabinose at 50℃ (26.265 ppm) whereas xylo-oligosaccharide at 50℃ (280.465 ppm).
AB - Indonesia is the largest country of oil palm production in the world. The oil palm by-product is still containing almost 80% of carbohydrates, such as cellulose and hemicellulose. This study aimed to optimize the enzymatic degradation of oil palm leaves in order to achieve zero waste of oil palm biomass by-product. Sodium hydroxide (NaOH) solution was used to isolate hemicellulose extraction from oil palm leaves. Various concentrations of NaOH such as 2 M, 2.5 M, 3 M, 3.5 M and 4 M and the reflux times of 2 hours, 4 hours, and 6 hours were used to optimize hemicellulose extraction. The optimum condition for hemicellulose extraction was 3 M NaOH and reflux time was 6 hours, while the yield at that condition was 48.94 % (w/w). The extracted hemicellulose is then hydrolyzed by a xylanolytic enzyme from recombinant Escherichia coli DH5α (namely pTP510). Temperatures of incubation were varied 50℃, 60℃, 70℃, and 80℃ for 24 hours at pH 6. The hydrolysis result was analyzed using High Performance Liquid Chromatography (HPLC). Xylose was produced at an optimum temperature of 60℃ (120.231 ppm), arabinose at 50℃ (26.265 ppm) whereas xylo-oligosaccharide at 50℃ (280.465 ppm).
UR - http://www.scopus.com/inward/record.url?scp=85146530453&partnerID=8YFLogxK
U2 - 10.1063/5.0111424
DO - 10.1063/5.0111424
M3 - Conference contribution
AN - SCOPUS:85146530453
T3 - AIP Conference Proceedings
BT - 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
A2 - Kristiana, Arika Indah
A2 - Alfarisi, Ridho
PB - American Institute of Physics Inc.
T2 - 1st International Conference on Neuroscience and Learning Technology, ICONSATIN 2021
Y2 - 18 September 2021 through 19 September 2021
ER -