TY - JOUR
T1 - OPG and RANKL Expression in Osteoblast Culture after Application of Osphronemus Gourami Fish Scale Collagen Peptide
AU - Wijaksana, I. Komang Evan
AU - Prahasanti, Chiquita
AU - Bargowo, Lambang
AU - Sukarsono, Rika Mutiasari
AU - Krismariono, Agung
N1 - Funding Information:
The authors would like to thank the Faculty of Dental Medicine Universitas Airlangga for its support of this research. Financial disclosure; The research was funded by Universitas Airlangga.
Publisher Copyright:
© 2021. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Periodontal disease can be treated with surgical procedures using bone graft. This research investigated the potency of Osphronemus gourami fish scales as bone graft material. Scaffold derived from collagen is employed in tissue engineering because of its biocompatibility, high porosity, low antigenicity, and ability to be resorbed by tissue. The study aims to analyse the potential effect of gourami fish-scale collagen peptide on expression of Osteoprotegerin (OPG) and Receptor Activator of Nuclear Factor-κB Ligand (RANKL) in osteoblast cell culture. This research constituted a study of rat calvaria osteoblasts by post-test only controls group design. Fish scale collagen derived from gourami was hydrolysed into collagen peptides, before being applied to osteoblasts culture. Application of fish scale collagen at concentrations of 0.0125 mg/ml, 0.05 mg/ml, 0.2 mg/ml, and 0.8 mg/ml in osteoblast culture was observed at 24 hours, 48 hours and 72 hours. Statistical analysis used a One Way Anova test with a significance level of 5%. Our study showed there was a significant difference in the expression of OPG at time observation intervals of 24 hours (p=0.002), 48 hours (p=0.001), and 72 hours (p=0.019) between groups. There is no significant difference in the expression of RANKL at an observation time. Gourami fish-scale collagen peptide administration on osteoblast cell culture enhance the expression of OPG and RANKL.
AB - Periodontal disease can be treated with surgical procedures using bone graft. This research investigated the potency of Osphronemus gourami fish scales as bone graft material. Scaffold derived from collagen is employed in tissue engineering because of its biocompatibility, high porosity, low antigenicity, and ability to be resorbed by tissue. The study aims to analyse the potential effect of gourami fish-scale collagen peptide on expression of Osteoprotegerin (OPG) and Receptor Activator of Nuclear Factor-κB Ligand (RANKL) in osteoblast cell culture. This research constituted a study of rat calvaria osteoblasts by post-test only controls group design. Fish scale collagen derived from gourami was hydrolysed into collagen peptides, before being applied to osteoblasts culture. Application of fish scale collagen at concentrations of 0.0125 mg/ml, 0.05 mg/ml, 0.2 mg/ml, and 0.8 mg/ml in osteoblast culture was observed at 24 hours, 48 hours and 72 hours. Statistical analysis used a One Way Anova test with a significance level of 5%. Our study showed there was a significant difference in the expression of OPG at time observation intervals of 24 hours (p=0.002), 48 hours (p=0.001), and 72 hours (p=0.019) between groups. There is no significant difference in the expression of RANKL at an observation time. Gourami fish-scale collagen peptide administration on osteoblast cell culture enhance the expression of OPG and RANKL.
KW - Collagen
KW - infectious Disease
KW - OPG
KW - Osphronemus gourami fish scales
KW - RANKL
UR - http://www.scopus.com/inward/record.url?scp=85110602057&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85110602057
SN - 1309-100X
VL - 14
SP - 618
EP - 622
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 2
ER -
