Periodontal disease can be treated with surgical procedures using bone graft. This research investigated the potency of Osphronemus gourami fish scales as bone graft material. Scaffold derived from collagen is employed in tissue engineering because of its biocompatibility, high porosity, low antigenicity, and ability to be resorbed by tissue. The study aims to analyse the potential effect of gourami fish-scale collagen peptide on expression of Osteoprotegerin (OPG) and Receptor Activator of Nuclear Factor-κB Ligand (RANKL) in osteoblast cell culture. This research constituted a study of rat calvaria osteoblasts by post-test only controls group design. Fish scale collagen derived from gourami was hydrolysed into collagen peptides, before being applied to osteoblasts culture. Application of fish scale collagen at concentrations of 0.0125 mg/ml, 0.05 mg/ml, 0.2 mg/ml, and 0.8 mg/ml in osteoblast culture was observed at 24 hours, 48 hours and 72 hours. Statistical analysis used a One Way Anova test with a significance level of 5%. Our study showed there was a significant difference in the expression of OPG at time observation intervals of 24 hours (p=0.002), 48 hours (p=0.001), and 72 hours (p=0.019) between groups. There is no significant difference in the expression of RANKL at an observation time. Gourami fish-scale collagen peptide administration on osteoblast cell culture enhance the expression of OPG and RANKL.

Original languageEnglish
Pages (from-to)618-622
Number of pages5
JournalJournal of International Dental and Medical Research
Issue number2
Publication statusPublished - 2021


  • Collagen
  • infectious Disease
  • OPG
  • Osphronemus gourami fish scales


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