TY - JOUR
T1 - Novel antiviral investigation of Annona squamosa leaf extract against the dengue virus type-2
T2 - In Vitro study
AU - Ansori, Arif Nur Muhammad
AU - Fadholly, Amaq
AU - Proboningrat, Annise
AU - Antonius, Yulanda
AU - Hayaza, Suhailah
AU - Susilo, Raden Joko Kuncoroningrat
AU - Inayatillah, Bilqis
AU - Sibero, Mada Triandala
AU - Naw, Sin War
AU - Posa, Gabrielle Ann Villar
AU - Sucipto, Teguh Hari
AU - Soegijanto, Soegeng
N1 - Publisher Copyright:
© 2021 Phcogj.Com.
PY - 2021/3
Y1 - 2021/3
N2 - Introduction: Dengue virus (DENV) infection is general mosquito-transmitted viral taint. It can lead to the dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue is a solemn illness with no endowed antiviral medication or recognized vaccine. Therefore, we aimed to investigate the activity of Annona squamosa leaf extract (ASLE) against dengue virus type-2 (DENV-2) isolated from Surabaya, Indonesia in 2013 (NCBI accession number: KT012509). Methods: In this study, the antiviral activity of ASLE was evaluated against DENV-2 in Vero cells using Viral ToxGlo™ Assay. In addition, we used CellTiter-Glo® Luminescent Cell Viability Assay to set the amount of viable cells in culture based on quantitation of the ATP. Results: DENV-2 replication inhibited by ASLE in Vero cells with IC50 = 73.78 μg/mL and SI = 4.49 when cells were treated two days after virus infection, whereas its CC50 for cytotoxicity to Vero cells was 331.54 μg/mL. Interestingly, this is the first report on the investigation of ASLE against DENV-2. Conclusion: In summary, ASLE demonstrated the antiviral activity against DENV-2 with less toxicity, and high possibility as a drug candidate. Therefore, it might be suggested for in vivo assessment in the progress of a potent antiviral against DENV-2.
AB - Introduction: Dengue virus (DENV) infection is general mosquito-transmitted viral taint. It can lead to the dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). Dengue is a solemn illness with no endowed antiviral medication or recognized vaccine. Therefore, we aimed to investigate the activity of Annona squamosa leaf extract (ASLE) against dengue virus type-2 (DENV-2) isolated from Surabaya, Indonesia in 2013 (NCBI accession number: KT012509). Methods: In this study, the antiviral activity of ASLE was evaluated against DENV-2 in Vero cells using Viral ToxGlo™ Assay. In addition, we used CellTiter-Glo® Luminescent Cell Viability Assay to set the amount of viable cells in culture based on quantitation of the ATP. Results: DENV-2 replication inhibited by ASLE in Vero cells with IC50 = 73.78 μg/mL and SI = 4.49 when cells were treated two days after virus infection, whereas its CC50 for cytotoxicity to Vero cells was 331.54 μg/mL. Interestingly, this is the first report on the investigation of ASLE against DENV-2. Conclusion: In summary, ASLE demonstrated the antiviral activity against DENV-2 with less toxicity, and high possibility as a drug candidate. Therefore, it might be suggested for in vivo assessment in the progress of a potent antiviral against DENV-2.
KW - Annona squamosa
KW - Antiviral activity
KW - DENV-2
KW - Vero cells
UR - http://www.scopus.com/inward/record.url?scp=85105555815&partnerID=8YFLogxK
U2 - 10.5530/pj.2021.13.58
DO - 10.5530/pj.2021.13.58
M3 - Article
AN - SCOPUS:85105555815
SN - 0975-3575
VL - 13
SP - 456
EP - 462
JO - Pharmacognosy Journal
JF - Pharmacognosy Journal
IS - 2
ER -
