Non-specific Effect (NSEs) of Supernatant L20B Culture Cell after OPV exposure against shigella flexneri adhesion to mice enterocyte

Introduction Besides protect against specific microorganisms, vaccines can induce heterologous or non-specific effects (NSE). As the global polio eradication initiative prepared to cease use of oral polio vaccine (OPV) in 2020, there is increasing interest in the understanding of oral vaccination provides non-specific immunity to other infections so the consequence of this transition can be adequately planned for and mitigated. Method This research was laboratory experimental research using Randomized Post Test Only Control Group design to prove the difference in adhesion of Shigella flexneri on Balb/c mice enterocytes after variety concentration administration of supernatant L20B Culture cell after OPV exposure. Supernatant concentrations of 0%, 12.5%, 25%, 50%, 100% each was coated to enterocytes Balb/c mice, then infected with Shigella flexneri. After six hours incubation at 37°C, a small volume of the mixture was made for Gram staining and examined microscopically under high power field. Shigella flexneri adhere to 100 enterocytes were counted and analyzed using correlation test and Anova. Result here were statistically difference in adhesion between 0% and 25%, 50% and 100%; 12.5% and 50% and 100%; 25% and 50% and 100%; 50% and 100%. There was a strong correlation between supernatant concentration and Shigella flexneri adhesion index in Balb/c mice enterocyte. Conclusion There were index differences of adhesion S flexneri on Balb/c mice enterocyte after giving L20B cell culture supernatant after OPV exposure which was meaningful differences between 0% with 25%, 50%, and 100%. There was strong correlation between supernatant concentration and Shigella flexneri adhesion index in Balb/c mice enterocyte.