Nf-Kb expressions on rat dental pulp mechanically exposured after pomegranate fruit extract administration

The Objective of this study was to analyze the activity of pomegranate extract on rat dental pulp mechanically exposured on the expression of NF-kB. Eighteen male Wistar rats aged 2.5 months and has a weight of about 190-230 grams were divided into 3 groups. The first group (A) was rat dental pulps mechanically exposured group which cappped with carboxy methyl cellulose (CMC-Na) 3% gel as control, the second group was cappped with calcium hydroxide(B), the third group was cappped with pomegranate fruit extract gel (C). The group consists of 6 rat each. The expression of NF-kB was examined using immunohistochemical techniques after one day of treatment. Data were analyzed using ANOVA and followed by HSD test. The Results showed that there were significance difference among the groups (p < 0.05). Pomegranate fruit extract as a pulp capping material were found to inhibit translocation of NF-kB into the nucleus of cell, it could be proved by lowering NF-kB expression. In Conclusion, these data indicate that pomegranate fruit extract decreased NF-Kb expresion and thus lowering the production of pro inflammatory cytokine so accelerate inflammation process.