TY - JOUR
T1 - New Methyl Threonolactones and Pyroglutamates of Spilanthes acmella (L.) L. And their bone formation activities
AU - Widyowati, Retno
AU - Sulistyowaty, Melanny Ika
AU - Uyen, Nguyen Hoang
AU - Sugimoto, Sachiko
AU - Yamano, Yoshi
AU - Otsuka, Hideaki
AU - Matsunami, Katsuyoshi
N1 - Funding Information:
This research was funded by PTUPT 2017 research grant of the Indonesia Government, JSPS KAKENHI grant nos. 17K08336, 16K18896, 17K15465, and 15H04651, and the cosmetology research foundation. The authors are grateful for access to the superconducting NMR instrument, UV, and ESI-MS at the Analytical Center of Molecular Medicine, the Analysis Center of Life Science, and the Natural Science Center for Basic Research and Development of the Graduate School of Biomedical and Health Sciences, Hiroshima University.
Funding Information:
Funding: This research was funded by PTUPT 2017 research grant of the Indonesia Government, JSPS KAKENHI grant nos. 17K08336, 16K18896, 17K15465, and 15H04651, and the cosmetology research foundation.
Publisher Copyright:
© 2020 by the authors.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - In our continuing research for bioactive constituents from natural resources, a new methyl threonolactone glucopyranoside (1), a new methyl threonolactone fructofuranoside (2), 2 new pyroglutamates (3 and 4), and 10 known compounds (5–14) were isolated from the whole plant of Spilanthes acmella (L.) L. The structures of these compounds were determined based on various spectroscopic and chemical analyses. All of the isolated compounds were evaluated on bone formation parameters, such as ALP (alkaline phosphatase) and mineralization stimulatory activities of MC3T3-E1 cell lines. The results showed that the new compound, 1,3-butanediol 3-pyroglutamate (4), 2-deoxy-D-ribono-1,4-lactone (6), methyl pyroglutamate (7), ampelopsisionoside (10), icariside B1 (11), and benzyl α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside (12) stimulated both ALP and mineralization activities.
AB - In our continuing research for bioactive constituents from natural resources, a new methyl threonolactone glucopyranoside (1), a new methyl threonolactone fructofuranoside (2), 2 new pyroglutamates (3 and 4), and 10 known compounds (5–14) were isolated from the whole plant of Spilanthes acmella (L.) L. The structures of these compounds were determined based on various spectroscopic and chemical analyses. All of the isolated compounds were evaluated on bone formation parameters, such as ALP (alkaline phosphatase) and mineralization stimulatory activities of MC3T3-E1 cell lines. The results showed that the new compound, 1,3-butanediol 3-pyroglutamate (4), 2-deoxy-D-ribono-1,4-lactone (6), methyl pyroglutamate (7), ampelopsisionoside (10), icariside B1 (11), and benzyl α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside (12) stimulated both ALP and mineralization activities.
KW - Alkaline phosphatase
KW - Methyl threonolactone
KW - Mineralization
KW - Pyroglutamate
KW - Spilanthes acmella
UR - http://www.scopus.com/inward/record.url?scp=85085676397&partnerID=8YFLogxK
U2 - 10.3390/molecules25112500
DO - 10.3390/molecules25112500
M3 - Article
C2 - 32481486
AN - SCOPUS:85085676397
SN - 1420-3049
VL - 25
JO - Molecules
JF - Molecules
IS - 11
M1 - 2500
ER -