TY - JOUR
T1 - New haplotypes of black-bearded tomb bat (Taphozous melanopogon) from puncakwangi cave (east java, indonesia)
AU - Amin, Muhammad Hilman Fu’Adil
AU - Nisa, Nabilatun
AU - Irawan, Bambang
N1 - Publisher Copyright:
© EM International.
PY - 2019
Y1 - 2019
N2 - DNA barcoding is a new method for identifying species from any biological species using standardized target gen and/or intergenic target. However, identification success is only as good as the underlying database. In this study, cytochrome c oxidase I (COI) sequences of 6 individuals were generated and analyzed from Taphozous melanopogon. Genetic distance, Neighbor-Joining (NJ) tree, and haplotype network analyses were performed to identify new haplotype. COI sequence analysis from the present study, combined with downloaded sequences from NCBI GenBank, revealed two new COI haplotypes from East Java, Indonesia. Population of T. melanopogon in East Java have unique haplotypes which are discrete from the haplotypes from Genbank (Myanmar/Vietnam) with pairwise distances 0.27-0.28 and number of segregating sites among samples are 19 nucleotides and number of parsimony-informative sites are 17 nucleotides, demonstrating that population in this study are highly isolated. The existence of isolated haplotypes highlights the significance of considering genetic diversity in developing sustainable management and conservation plans for biodiversity.
AB - DNA barcoding is a new method for identifying species from any biological species using standardized target gen and/or intergenic target. However, identification success is only as good as the underlying database. In this study, cytochrome c oxidase I (COI) sequences of 6 individuals were generated and analyzed from Taphozous melanopogon. Genetic distance, Neighbor-Joining (NJ) tree, and haplotype network analyses were performed to identify new haplotype. COI sequence analysis from the present study, combined with downloaded sequences from NCBI GenBank, revealed two new COI haplotypes from East Java, Indonesia. Population of T. melanopogon in East Java have unique haplotypes which are discrete from the haplotypes from Genbank (Myanmar/Vietnam) with pairwise distances 0.27-0.28 and number of segregating sites among samples are 19 nucleotides and number of parsimony-informative sites are 17 nucleotides, demonstrating that population in this study are highly isolated. The existence of isolated haplotypes highlights the significance of considering genetic diversity in developing sustainable management and conservation plans for biodiversity.
KW - COI
KW - Chiroptera
KW - DNA Barcoding
KW - New haplotype
KW - Taphozous melanopogon
UR - http://www.scopus.com/inward/record.url?scp=85071751081&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85071751081
SN - 0971-765X
VL - 25
SP - S194-S198
JO - Ecology, Environment and Conservation
JF - Ecology, Environment and Conservation
IS - 7
ER -