New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering

Maretaningtias Dwi Ariani, Ayumu Matsuura, Isao Hirata, Takayasu Kubo, Koichi Kato, Yasumasa Akagawa

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Carbonate apatite-chitosan scaffolds (CA-ChSs) were fabricated using the lyophilization technique. It was found that ChSs prepared with 200 mg chitosan powder (ChSs200) had well-structured three-dimensional architecture with high porosity and good retentive form without brittleness. In addition, it was shown that the number of osteoblast-like cells MC3T3-E1 proliferated on desalinated ChSs200 was larger than that on the non-desalinated ChSs200. CA-ChSs were fabricated by adding 100 mg carbonate apatite (CA) to 200 mg chitosan gels followed by freeze-drying (CA100ChSs200). SEM observation revealed that CA100ChSs200 had favorable threedimensional porous structures. The number of living cells increased more rapidly on CA100ChSs200 prepared with different amounts of CA than on ChSs. ALP activity significantly increased after day 14 and reached a plateau after day 21 in ChSs200 and CA100ChSs200. It was concluded that newly developed CA100ChSs200 may be a possible scaffold material for tissue engineering.

Original languageEnglish
Pages (from-to)317-325
Number of pages9
JournalDental Materials Journal
Volume32
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • Carbonate apatite
  • Cell proliferation
  • Chitosan
  • Scaffold
  • Tissue engineering

Fingerprint

Dive into the research topics of 'New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering'. Together they form a unique fingerprint.

Cite this