TY - JOUR
T1 - Necroptosis after lipopolysaccharide administration based on expression of mixed lineage kinase domain-like protein and number of nerve cells in dental pulp tissue
AU - Sampoerno, Galih
AU - Saraswati, Widya
AU - Shabrina, Hasna
AU - Fripertiwi, Nathania Nurani
AU - Fauzya, Sarah Aniqoh
N1 - Publisher Copyright:
© 2024 by SPC.
PY - 2024/2
Y1 - 2024/2
N2 - In dental pulp tissue, lipopolysaccharide invasion may be the cause of nerve cell death, which lowers the total amount of nerve cells in the pulp. The disease causes the pulp of teeth to deteriorate, which may have an impact on the risk of endodontic flare-ups. One sort of cell death used to lower the quantity of undesirable cells that might compromise pulp quality is called necroptosis. The goal of this study is to understand how Porphyromonas gingivalis lipopolysaccharide injection causes the necroptosis of dental pulp nerve cells. For experimental section, 32 Sprague Dawley rats in total were divided into 2 groups. Lower incisor access was induced in the treatment group using Porphyromonas gingivalis lipopolysaccharide, whereas in the control group, lower incisor access was performed and filled with GIC. A day later, both groups were closed. Next, preparation slides were made, and using immunohistochemical labeling, the number of nerve cells and Mixed Lineage Kinase Domain-Like Protein (MLKL) was measured. The findings demonstrate a substantial difference between the lipopolysaccharide treatment group and the control group, as well as an increase in MLKL expression and a decrease in nerve cell count following lipopolysaccharide injection. Finally, this study shows that the injection of Porphyromonas gingivalis lipopolysaccharide results in necroptosis in dental pulp tissue nerve cells.
AB - In dental pulp tissue, lipopolysaccharide invasion may be the cause of nerve cell death, which lowers the total amount of nerve cells in the pulp. The disease causes the pulp of teeth to deteriorate, which may have an impact on the risk of endodontic flare-ups. One sort of cell death used to lower the quantity of undesirable cells that might compromise pulp quality is called necroptosis. The goal of this study is to understand how Porphyromonas gingivalis lipopolysaccharide injection causes the necroptosis of dental pulp nerve cells. For experimental section, 32 Sprague Dawley rats in total were divided into 2 groups. Lower incisor access was induced in the treatment group using Porphyromonas gingivalis lipopolysaccharide, whereas in the control group, lower incisor access was performed and filled with GIC. A day later, both groups were closed. Next, preparation slides were made, and using immunohistochemical labeling, the number of nerve cells and Mixed Lineage Kinase Domain-Like Protein (MLKL) was measured. The findings demonstrate a substantial difference between the lipopolysaccharide treatment group and the control group, as well as an increase in MLKL expression and a decrease in nerve cell count following lipopolysaccharide injection. Finally, this study shows that the injection of Porphyromonas gingivalis lipopolysaccharide results in necroptosis in dental pulp tissue nerve cells.
KW - MLKL
KW - Necroptosis
KW - immunology
KW - lipopolysaccharide
KW - nerve cells
UR - http://www.scopus.com/inward/record.url?scp=85178239563&partnerID=8YFLogxK
U2 - 10.48309/jmpcr.2024.184113
DO - 10.48309/jmpcr.2024.184113
M3 - Article
AN - SCOPUS:85178239563
SN - 2981-0221
VL - 6
SP - 236
EP - 244
JO - Journal of Medicinal and Pharmaceutical Chemistry Research
JF - Journal of Medicinal and Pharmaceutical Chemistry Research
IS - 2
ER -