TY - JOUR
T1 - Nanocurcumin protective effect on lipid peroxide of lead acetate induced white rats (Rattus norvegicus) ovarian granulosa cells
AU - Syarifah, Anis Satus
AU - Sudjarwo, Sri Agus
AU - Hendarto, Hendy
AU - I’tishom, Reny
N1 - Publisher Copyright:
© 2020, Institute of Medico-Legal Publications. All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Objective: This study aims to investigate the protective effect of nanocurcumin on lipid peroxidation of rat ovarian granulosa cells induced by lead acetate (Pb). Methods: A total of 45 mice were divided into a negative control group (C-) receiving corn oil, a positive control group (C+) receiving corn oil and lead acetate (Pb) 40 mg/kg bw/day, experimental group 1 (E1), experimental group 2 (E2), experimental group 3 (E3) receiving 50, 100 and 200 mg/kg/day nanocurcumin dissolved in corn oil and lead acetate (Pb) 40 mg/kg bw/day. The treatment was given orally and the treatment time was daily at 08.00 AM for 26 days. Ovaries were removed for immunohistochemical analysis of MDA expression. Result: The positive control group had the highest MDA expression of ovarian granulosa cells (94.2 ± 10.5) and was significantly different (p <0.05) from negative control group (1.5 ± 1.4), experimental group 1 (17.4 ± 10.5), experimental group 2 (11.4 ± 8.5) and experimental group 3 (7.2 ± 4.0). Conclusion: Nanocurcumin as a protective oxidative stress on ovarian granulosa cells due to exposure to lead acetate, by protecting lipid peroxidation.
AB - Objective: This study aims to investigate the protective effect of nanocurcumin on lipid peroxidation of rat ovarian granulosa cells induced by lead acetate (Pb). Methods: A total of 45 mice were divided into a negative control group (C-) receiving corn oil, a positive control group (C+) receiving corn oil and lead acetate (Pb) 40 mg/kg bw/day, experimental group 1 (E1), experimental group 2 (E2), experimental group 3 (E3) receiving 50, 100 and 200 mg/kg/day nanocurcumin dissolved in corn oil and lead acetate (Pb) 40 mg/kg bw/day. The treatment was given orally and the treatment time was daily at 08.00 AM for 26 days. Ovaries were removed for immunohistochemical analysis of MDA expression. Result: The positive control group had the highest MDA expression of ovarian granulosa cells (94.2 ± 10.5) and was significantly different (p <0.05) from negative control group (1.5 ± 1.4), experimental group 1 (17.4 ± 10.5), experimental group 2 (11.4 ± 8.5) and experimental group 3 (7.2 ± 4.0). Conclusion: Nanocurcumin as a protective oxidative stress on ovarian granulosa cells due to exposure to lead acetate, by protecting lipid peroxidation.
KW - Lead acetate
KW - Malondialdehyde (MDA)
KW - Nanocurcumin
UR - http://www.scopus.com/inward/record.url?scp=85097816759&partnerID=8YFLogxK
U2 - 10.37506/ijfmt.v14i4.11581
DO - 10.37506/ijfmt.v14i4.11581
M3 - Article
AN - SCOPUS:85097816759
SN - 0973-9122
VL - 14
SP - 764
EP - 769
JO - Indian Journal of Forensic Medicine and Toxicology
JF - Indian Journal of Forensic Medicine and Toxicology
IS - 4
ER -