Nanocurcumin Protective Effect on Gpx Scavenger Enzyme Expression and Apoptosis of Lead Acetate-Induced Rats Ovarian Granulosa Cells

This study aims to prove the protective effect of nanocurcumin against the reduction of glutathione peroxidase (GPx) scavenger enzymeand the increase of apoptosis in lead acetate-induced rat ovarian granulosa cells. A total of 45 female white rats were divided into 5 groups, the negative control group (receiving corn oil and one hour later receiving distilled water), positive control group (receiving corn oil and one hour later receiving lead acetate (Pb) of 40 mg/kg bw), and experimental groups 1, 2 and 3 (receiving nanocurcumin 50, 100 and 200 mg/kg, and one hour later receiving lead acetate (Pb) 40 mg/kg bw). All groups received treatments per oral once a day for 26 days. On day 27 the rats were sacrificed and then the expression of the GPx scavenger enzyme and apoptosis was performed by immunohistochemistry. Lead acetate (Pb) decreased the expression of the GPx scavenger enzyme and increased apoptosis. The administration of nanocurcumin increased the expression of GPx scavenger enzyme and decreased the expression of apoptosis in lead acetate-induced rat ovarian granulosa cells. Nanocurcumin functions as an antioxidant by providing protective effect against GPx scavenger enzyme and apoptosis in lead acetate (Pb)-induced rat granulosa cells.