Nano spray inhaler ashitaba leaf extract (Angelica keiskei) on malondialdehyde, catalase enzyme activity and lung tissue damage in mice exposed to cigarette smoke

A total of 30 mice were divided into 5 treatment groups. K1: control (-) group not exposed to cigarette smoke and not given nanospray inhalers. K2: control (+) group exposed to cigarette smoke and not given nanospray inhalers. P1, P2, and P3 The treatment group exposed to cigarette smoke for 28 days and given nanospray inhaler asitaba leaf extract (Angelica keiskei) at a dose of 50 g / KgBB / day, 200 g / KgBB / day and 500 g / KgBB /day. Day 29 was given nanospray inhalers for 3 weeks, then Malondialdehyde (MDA), enzyme catalase activity ware examined from serum and lung organs for pulmonary histopathology. The administration of 200 g / KgBB / day nanospray inhaler can reduce malondialdehyde levels, activation of the enzyme catalase, emphysema, inflammatory cell infiltration and the number of erythrocytes exposed to cigarette smoke.