Nano-emulsion of mangosteen rind extract in a mucoadhesive patch for periodontitis regenerative treatment: An in vivo study

Mohammed A. Aljuanid, Huda R. Qaid, Dur M. Lashari, Rini D. Ridwan, Hendrik S. Budi, Baleegh A. Alkadasi, Yeka Ramadhani, Riski Rahmasari

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Objective: To investigate the therapeutic potential of nano-emulsion of mangosteen rind extract in a mucoadhesive gingival patch on periodontitis, and its effect on tumor necrosis factor alpha (TNF-α), receptor activator of nuclear factor kappa Β ligand (RANKL), and interleukin 10 (IL-10) expression. Methods: Sixty Wistar rats were divided into four groups: positive control group (mucoadhesive patch with doxycycline), negative control group (mucoadhesive patch), treatment group I (mucoadhesive patch with mangosteen rind extract), and treatment group II (mucoadhesive patch with nano-emulsion of mangosteen rind extract). An experimental model of Porphyromonas gingivalis-induced periodontitis was established in rats by treatment with 0.03 mL bacteria locally (1 × 1010 colony-forming units) seven times at 2-day intervals in the gingival sulcus of mandibular anterior teeth. Treatment was 1 h/day for 3 days. On days 3, 5, and 7, five rats from each group were killed. TNF-α, IL-10, and RANKL expression was determined by dissecting the lower jaw for immunohistochemistry. Results: The mucoadhesive patch with nano-emulsion mangosteen rind extract significantly decreased TNF-α and RANKL expression and increased IL-10 expression (p < 0.05) compared to the treatment I, positive and negative control groups. Conclusion: A mucoadhesive gingival patch with nano-emulsion of mangosteen rind extract has the potential to treat periodontitis by decreasing TNF-α, RANKL, and increasing IL-10 expression.

Original languageEnglish
Pages (from-to)910-920
Number of pages11
JournalJournal of Taibah University Medical Sciences
Volume17
Issue number5
DOIs
Publication statusPublished - Oct 2022

Keywords

  • Mangosteen rind extract
  • Mucoadhesive gingival patch
  • Nano-emulsion
  • P. gingivalisbacteria
  • Periodontitis

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