TY - JOUR
T1 - Nano-emulsion of mangosteen rind extract in a mucoadhesive patch for periodontitis regenerative treatment
T2 - An in vivo study
AU - Aljuanid, Mohammed A.
AU - Qaid, Huda R.
AU - Lashari, Dur M.
AU - Ridwan, Rini D.
AU - Budi, Hendrik S.
AU - Alkadasi, Baleegh A.
AU - Ramadhani, Yeka
AU - Rahmasari, Riski
N1 - Publisher Copyright:
© 2022 [The Author/The Authors]
PY - 2022/10
Y1 - 2022/10
N2 - Objective: To investigate the therapeutic potential of nano-emulsion of mangosteen rind extract in a mucoadhesive gingival patch on periodontitis, and its effect on tumor necrosis factor alpha (TNF-α), receptor activator of nuclear factor kappa Β ligand (RANKL), and interleukin 10 (IL-10) expression. Methods: Sixty Wistar rats were divided into four groups: positive control group (mucoadhesive patch with doxycycline), negative control group (mucoadhesive patch), treatment group I (mucoadhesive patch with mangosteen rind extract), and treatment group II (mucoadhesive patch with nano-emulsion of mangosteen rind extract). An experimental model of Porphyromonas gingivalis-induced periodontitis was established in rats by treatment with 0.03 mL bacteria locally (1 × 1010 colony-forming units) seven times at 2-day intervals in the gingival sulcus of mandibular anterior teeth. Treatment was 1 h/day for 3 days. On days 3, 5, and 7, five rats from each group were killed. TNF-α, IL-10, and RANKL expression was determined by dissecting the lower jaw for immunohistochemistry. Results: The mucoadhesive patch with nano-emulsion mangosteen rind extract significantly decreased TNF-α and RANKL expression and increased IL-10 expression (p < 0.05) compared to the treatment I, positive and negative control groups. Conclusion: A mucoadhesive gingival patch with nano-emulsion of mangosteen rind extract has the potential to treat periodontitis by decreasing TNF-α, RANKL, and increasing IL-10 expression.
AB - Objective: To investigate the therapeutic potential of nano-emulsion of mangosteen rind extract in a mucoadhesive gingival patch on periodontitis, and its effect on tumor necrosis factor alpha (TNF-α), receptor activator of nuclear factor kappa Β ligand (RANKL), and interleukin 10 (IL-10) expression. Methods: Sixty Wistar rats were divided into four groups: positive control group (mucoadhesive patch with doxycycline), negative control group (mucoadhesive patch), treatment group I (mucoadhesive patch with mangosteen rind extract), and treatment group II (mucoadhesive patch with nano-emulsion of mangosteen rind extract). An experimental model of Porphyromonas gingivalis-induced periodontitis was established in rats by treatment with 0.03 mL bacteria locally (1 × 1010 colony-forming units) seven times at 2-day intervals in the gingival sulcus of mandibular anterior teeth. Treatment was 1 h/day for 3 days. On days 3, 5, and 7, five rats from each group were killed. TNF-α, IL-10, and RANKL expression was determined by dissecting the lower jaw for immunohistochemistry. Results: The mucoadhesive patch with nano-emulsion mangosteen rind extract significantly decreased TNF-α and RANKL expression and increased IL-10 expression (p < 0.05) compared to the treatment I, positive and negative control groups. Conclusion: A mucoadhesive gingival patch with nano-emulsion of mangosteen rind extract has the potential to treat periodontitis by decreasing TNF-α, RANKL, and increasing IL-10 expression.
KW - Mangosteen rind extract
KW - Mucoadhesive gingival patch
KW - Nano-emulsion
KW - P. gingivalisbacteria
KW - Periodontitis
UR - http://www.scopus.com/inward/record.url?scp=85127531385&partnerID=8YFLogxK
U2 - 10.1016/j.jtumed.2022.03.003
DO - 10.1016/j.jtumed.2022.03.003
M3 - Article
AN - SCOPUS:85127531385
SN - 1658-3612
VL - 17
SP - 910
EP - 920
JO - Journal of Taibah University Medical Sciences
JF - Journal of Taibah University Medical Sciences
IS - 5
ER -