Mung Bean Flour Increases Blood Folate Levels in Pregnant Mice Model of Cleft Lip and Palate

To analyse mung bean flour (MBF) administration to increase blood folate levels in pregnant mice models of cleft lip with or without cleft palate (CL/P). An experimental study was conducted using Balb/C mice. Pregnant mice were randomly separated into five groups. The mice that received distilled water were used as negative control (C-), while mice in the other four groups (one positive control group (C+) and three treatment groups) were given with phenytoin of 60 mg/kg at gestational day (GD) 10 intraperitoneally and the three treatment groups were added with MBF 0.25 g, 0.5 g and 1 g/day, respectively. The MBF was given from GD 0 until the sampling data were collected at GD 17. Blood folate levels in T1 (0.25 g/d MBF), T2 (0.5 g/d MBF) and T3 (1 g/d MBF) groups were higher than those in C-and C+ groups. Anova test results on the comparison of the effect of MBF on blood folate levels in mice showed a significant difference with p = 0.037 (p <0.05). There was a significant difference the effect of MBF between C-and T1 (0.25 g/d MBF) groups, between C-and T3 (1 g/d MBF) groups, between C + and T1 (0.25 g/d MBF) groups, and between C + and T3 (1 g/d MBF) groups, but there was no significant difference between the C-and C + groups, and between T1 (0.25 g/d MBF), T2 (0.5 g/d MBF) and T3 (1 g/d MBF). Mung bean flour administration could increase blood folate levels in mother mice models of CL/P.