Monitoring Antibodies Against FMD Using ELISA in Vaccinated and Unvaccinated Cattle in Gresik Regency, Indonesia

Background: Indonesia is currently experiencing a Foot and Mouth Disease (FMD) outbreak in livestock caused by FMD virus. The Indonesian government has implemented a vaccination program for all FMD-susceptible animals using imported vaccines. This study aimed to determine the presence of antibodies against vaccination and field infections by examining SP-O and NSP antibodies. Methodology: Cattles serum samples were collected in Gresik Regency after the first booster vaccine, were tested using ELISA structural protein O and ELISA non structural protein at the National Center for Veterinary Biologics, Indonesia. Results: The results of 325 serum samples showed, that the SP-O antibody seroprevalence was 93.8%, whereas it was 100% in the vaccinated cattle samples and 50% in unvaccinated cattle. The results of the NSP antibody test showed that seroprevalence of FMD was 14.19% and 7.71% in vaccinated, and 60% in unvaccinated cattle. Conclusion: The study showed that vaccination can induce SP-O antibodies up to 100% in cattle in Gresik, the seroprevalence of FMD infection in cattle in Gresik was 14.19%, regional differences and vaccination significantly affected the prevalence of SP-O antibodies, age, region, and vaccination had a significant effect on NSP antibodies.