Molecular identification of the worm Fasciola sp. on cattle at the Kediri City Slaughterhouse, Indonesia

Fasciolosis is a disease caused by the genus Fasciola which attacks wild animals, livestock and humans. Molecular characterization of Fasciola sp. important for identifying the species Fasciola sp. The aim of this research was to analyze the characteristics of the worm Fasciola sp. molecularly on samples of cattle slaughtered at the Kediri City Slaughterhouse Regional Technical Implementation Unit. This research was carried out from January to February 2023. The samples were adult worms Fasciola sp. taken from one beef cattle and one dairy cattle. The PCR used is conventional PCR with primers from mitochondrial DNA genes. PCR products are passed to the sequencing stage. The PCR test results read on 2% agarose gel electrophoresis showed that the PCR product had high specificity, namely forming a single band at position 752 bp showed positive for F. gigantica. the results of the phylogenetic tree of Fasciola sp. with data in GenBank showing Fasciola sp. beef and dairy cattle isolates in Kediri City are closely related to Fasciola sp. dairy cattle isolate from China with Accession Number KF543343.1 and Fasciola sp. long-haired cattle (yak) isolate from China Accession Number MH621335.1. The existence of international livestock trade can result in the introduction of various diseases, one of which is Fasciolosis. Livestock import-export activities from China allow the introduction of Fasciola sp. to Indonesia.