Molecular identification of Eimeria hestermani and Eimeria prionotemni from a red-necked wallaby (Macropodidae; Macropus rufogriseus) in Japan

Fitrine Ekawasti, Kazuya Kitagawa, Hiroshi Domae, April Hari Wardhana, Tomoyuki Shibahara, Shigehiko Uni, Masaharu Tokoro, Kazumi Sasai, Makoto Matsubayashi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

To date, more than 50 Eimeria spp. have been isolated from marsupials of the family Macropodidae. Although 18 species of Eimeria have been previously detected from multiple animal species belonging to the genus Macropus of the family, limited genetic analyses of the parasites are available, and their pathogenicity remains unclear. Here, we report the isolation of Eimeria spp. from a zoo specimen of red-necked wallaby (Macropodidae; Macropus rufogriseus). Specifically, two distinct types of Eimeria oocysts were recovered, one from the feces before treatment with an anthelmintic and the second from the intestinal contents after death of the animal. The oocysts obtained from the two sources were morphologically identified as E. hestermani and E. prionotemni, respectively. We successfully determined partial gene sequences from the two isolates, including segments of the 18S rRNA genes, and for the first time have used phylogenetic analyses of these sequences to assign the species to distinct clades. In combination with further genetic data, these results are expected to help elucidate the pathogenicity and host ranges of Eimeria spp. within the respective family and genus.

Original languageEnglish
Pages (from-to)1271-1279
Number of pages9
JournalParasitology Research
Volume119
Issue number4
DOIs
Publication statusPublished - 1 Apr 2020

Keywords

  • Eimeria hestermani
  • Eimeria prionotemni
  • Japan
  • Macropodidae
  • Red-necked wallaby

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