TY - JOUR
T1 - Molecular detection of encoding enterotoxin C gene and profile of antibiotic resistant on staphylococcus aureus isolated from several dairy farms in East Java, Indonesia
AU - Harijani, Nenny
AU - Wandari, Ayu
AU - Effendi, Mustofa Helmi
AU - Tyasningsih, Wiwiek
N1 - Funding Information:
Twenty four Staphylococcus aureus isolates could be isolated from raw milk of dairy cows in several farms from East Java, Indonesia. One S. aureus was classified as MRSA bacteria. Through PCR testing, Enterotoxin C encoding gene of S. aureus could be identified in two isolates. The presence of Enterotoxin C encoding gene in S. aureus has the potential to spread its virulence factor to impact on the human health. ACKNOWLEDGEMENT This study was supported in part with the Hibah Mandat Universitas Airlangga Funds from Airlangga University, Indonesia. REFERENCES
Publisher Copyright:
© 2020 Connect Journal.
PY - 2020
Y1 - 2020
N2 - The purpose of this study were to identify the encoding gene for enterotoxin C and study the profile of antibiotic resistanton Staphylococcus aureus from raw milk in East Java, Indonesia. Raw milk samples of 148 samples obtained from four dairy farms. Bacterial identification was based on the growth in Mannitol Salt Agar (MSA) and Gram staining and catalase & coagulase tests. 24(16.2%) out of 148 milk samples were for positive Staphylococcus aureus isolation. Antibiotic sensitivity testing using Cefoxitin, Penicillin, Amphicillin, Erythromycin and Tetracyclin antibiotics showed 1(4%), 17(71%), 16(67%), 24 (100%) and 8(33%) isolates were resistant to the antibiotics, respectively. MRSA isolate showed that 1 isolate was positive by using Cefoxitin disc diffusion test. The molecular identification on enterotoxin C gene by PCR showed that 2(8%) isolates were positive contain enterotoxin C gene. It was concluded that the raw milk can be a potential reservoir for Staphylococcus aureus strains to threat public health.
AB - The purpose of this study were to identify the encoding gene for enterotoxin C and study the profile of antibiotic resistanton Staphylococcus aureus from raw milk in East Java, Indonesia. Raw milk samples of 148 samples obtained from four dairy farms. Bacterial identification was based on the growth in Mannitol Salt Agar (MSA) and Gram staining and catalase & coagulase tests. 24(16.2%) out of 148 milk samples were for positive Staphylococcus aureus isolation. Antibiotic sensitivity testing using Cefoxitin, Penicillin, Amphicillin, Erythromycin and Tetracyclin antibiotics showed 1(4%), 17(71%), 16(67%), 24 (100%) and 8(33%) isolates were resistant to the antibiotics, respectively. MRSA isolate showed that 1 isolate was positive by using Cefoxitin disc diffusion test. The molecular identification on enterotoxin C gene by PCR showed that 2(8%) isolates were positive contain enterotoxin C gene. It was concluded that the raw milk can be a potential reservoir for Staphylococcus aureus strains to threat public health.
KW - Antibiotic sensitivity test
KW - Enterotoxin C gene
KW - MRSA
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85090487870&partnerID=8YFLogxK
U2 - 10.35124/bca.2020.20.S1.3081
DO - 10.35124/bca.2020.20.S1.3081
M3 - Article
AN - SCOPUS:85090487870
SN - 0972-5075
VL - 20
SP - 3081
EP - 3085
JO - Biochemical and Cellular Archives
JF - Biochemical and Cellular Archives
ER -
