TY - JOUR
T1 - Molecular characterization of highly pathogenic Eimeria species among beef cattle on Java Island, Indonesia
AU - Ekawasti, Fitrine
AU - Nurcahyo, W.
AU - Wardhana, April Hari
AU - Shibahara, Tomoyuki
AU - Tokoro, Masaharu
AU - Sasai, Kazumi
AU - Matsubayashi, Makoto
N1 - Funding Information:
The authors gratefully acknowledge Mrs. Rika Sekiguchi and Mrs. Noriko Asama for helping in fecal and molecular examinations. Part of this study was supported by grants from Grants-in-Aid for Japan Society for the Promotion of Science (JSPS) (Grant No. 16H05803 ) and RTA Scheme Gadjah Mada University (Grant No. 130 ).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10
Y1 - 2019/10
N2 - Gastrointestinal parasites including Eimeria spp. are known to affect domestic animal productivity causing watery or lethal bloody diarrhea. However, there are few reports on the detailed distribution of bovine Eimeria spp. in cattle, particularly in developing tropical and sub-tropical areas. Using a total of 289 fecal samples collected from beef cattle on Java Island, one of the five main islands of Indonesia, fecal examinations by the Whitlock and sugar flotation methods and molecular surveys were conducted to reveal the prevalence of 6 Eimeria spp. As a result of morphological screening using Whitlock methods and sugar flotation, Eimeria spp. prevalences of 9.4% and 52.3% were confirmed, respectively. The prevalence was higher in younger cattle [under 1 year (63.9%), 1–2 years (75.0%) and more than in 2 year old cattle (42.3%)]. The prevalences of identified species were as follows: 10.4% for E. bovis, 2.8% for E. ellipsoidalis, 2.1% for E. alabamensis, 1.4% for E. zuernii, 1.1% for E. auburnensis, and 0.4% for E. cylindrica. Moreover, prevalences of 12.8% for Strongyloides papillosus, 7.3% for Trichuris globulosa, and 0.3% for Capillaria bovis were detected. Although the average number of oocysts per gram of feces was <100 among the positive samples, and cases of heavy infection were limited, the endemicity of these pathogenic Eimeria species among farms in Indonesia should be noted.
AB - Gastrointestinal parasites including Eimeria spp. are known to affect domestic animal productivity causing watery or lethal bloody diarrhea. However, there are few reports on the detailed distribution of bovine Eimeria spp. in cattle, particularly in developing tropical and sub-tropical areas. Using a total of 289 fecal samples collected from beef cattle on Java Island, one of the five main islands of Indonesia, fecal examinations by the Whitlock and sugar flotation methods and molecular surveys were conducted to reveal the prevalence of 6 Eimeria spp. As a result of morphological screening using Whitlock methods and sugar flotation, Eimeria spp. prevalences of 9.4% and 52.3% were confirmed, respectively. The prevalence was higher in younger cattle [under 1 year (63.9%), 1–2 years (75.0%) and more than in 2 year old cattle (42.3%)]. The prevalences of identified species were as follows: 10.4% for E. bovis, 2.8% for E. ellipsoidalis, 2.1% for E. alabamensis, 1.4% for E. zuernii, 1.1% for E. auburnensis, and 0.4% for E. cylindrica. Moreover, prevalences of 12.8% for Strongyloides papillosus, 7.3% for Trichuris globulosa, and 0.3% for Capillaria bovis were detected. Although the average number of oocysts per gram of feces was <100 among the positive samples, and cases of heavy infection were limited, the endemicity of these pathogenic Eimeria species among farms in Indonesia should be noted.
KW - Cattle
KW - Eimeria bovis
KW - Eimeria zuernii
KW - Indonesia
KW - Java Island
UR - http://www.scopus.com/inward/record.url?scp=85068376510&partnerID=8YFLogxK
U2 - 10.1016/j.parint.2019.101927
DO - 10.1016/j.parint.2019.101927
M3 - Article
C2 - 31108220
AN - SCOPUS:85068376510
SN - 1383-5769
VL - 72
JO - Parasitology International
JF - Parasitology International
M1 - 101927
ER -