TY - JOUR
T1 - Metagenome and Resistome Analysis of Beta-Lactam-Resistant Bacteria Isolated from River Waters in Surabaya, Indonesia
AU - Nomoto, Ryohei
AU - Osawa, Kayo
AU - Kinoshita, Shohiro
AU - Kitagawa, Koichi
AU - Nakanishi, Noriko
AU - Sarassari, Rosantia
AU - Raharjo, Dadik
AU - Fujisawa, Masato
AU - Kuntaman, Kuntaman
AU - Shirakawa, Toshiro
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/1
Y1 - 2024/1
N2 - Antimicrobial agents are administered to humans and livestock, and bacterial antimicrobial resistance (AMR) and antimicrobial agents are released into the environment. In this study, to investigate the trend of AMR in humans, livestock, and the environment, we performed a metagenomic analysis of multidrug-resistant bacteria with CHROMagar ESBL in environmental river water samples, which were collected using syringe filter units from waters near hospitals, downtown areas, residential areas, and water treatment plants in Surabaya, Indonesia. Our results showed that Acinetobacter, Pseudomonas, Aeromonas, Enterobacter, Escherichia, and Klebsiella grew in CHROMagar ESBL; they were most frequently detected in water samples from rivers surrounding hospitals contaminated with various AMR genes (ARGs) in high levels. These results identified bacteria as ARG reservoirs and revealed that hospitals could be sources for various ARGs disseminated into the environment. In conclusion, this study details a novel metagenomic analysis of collected bacteria in environmental water samples using a syringe filter unit for an AMR epidemiological study based on the One Health approach.
AB - Antimicrobial agents are administered to humans and livestock, and bacterial antimicrobial resistance (AMR) and antimicrobial agents are released into the environment. In this study, to investigate the trend of AMR in humans, livestock, and the environment, we performed a metagenomic analysis of multidrug-resistant bacteria with CHROMagar ESBL in environmental river water samples, which were collected using syringe filter units from waters near hospitals, downtown areas, residential areas, and water treatment plants in Surabaya, Indonesia. Our results showed that Acinetobacter, Pseudomonas, Aeromonas, Enterobacter, Escherichia, and Klebsiella grew in CHROMagar ESBL; they were most frequently detected in water samples from rivers surrounding hospitals contaminated with various AMR genes (ARGs) in high levels. These results identified bacteria as ARG reservoirs and revealed that hospitals could be sources for various ARGs disseminated into the environment. In conclusion, this study details a novel metagenomic analysis of collected bacteria in environmental water samples using a syringe filter unit for an AMR epidemiological study based on the One Health approach.
KW - antimicrobial resistance genes (ARGs)
KW - antimicrobial-resistant bacteria (AMR)
KW - environmental river
KW - public health
KW - whole genome sequencing (WGS)
UR - http://www.scopus.com/inward/record.url?scp=85183642023&partnerID=8YFLogxK
U2 - 10.3390/microorganisms12010199
DO - 10.3390/microorganisms12010199
M3 - Article
AN - SCOPUS:85183642023
SN - 2076-2607
VL - 12
JO - Microorganisms
JF - Microorganisms
IS - 1
M1 - 199
ER -
