TY - JOUR
T1 - Metabolite profiling of Andrographis paniculata leaves and stem extract using UHPLC-Orbitrap-MS/MS
AU - Rafi, Mohamad
AU - Karomah, Alfi Hudatul
AU - Heryanto, Rudi
AU - Septaningsih, Dewi Anggraini
AU - Kusuma, Wisnu Ananta
AU - Amran, Muhammad Bachri
AU - Rohman, Abdul
AU - Prajogo, Bambang
N1 - Funding Information:
The authors gratefully acknowlegded Ministry of Research, Technology, and Higher Education of the Republic of Indonesia through World Class University Program of IPB University for financial support of this research by Riset Kolaborasi Indonesia Grant 2019 (No: 0847/IT3.L1/PN/2019).
Publisher Copyright:
© 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2020
Y1 - 2020
N2 - Andrographis paniculata is known as the king of bitter and it has been widely used as a medicinal plant. The properties of A. paniculata are generally determined by the metabolite composition, which may be influenced by several factors, one of which is the part of the plant extracted. The objectives of this research are to identify putatively the metabolite composition of the stem and the leaves extracts using LC-MS/MS and classify them using PCA. The stem and the leaves samples were separated and extracted using sonication with 70% ethanol. A total of 31 metabolite compounds has been putatively identified. All compounds were identified in the stem and the leaves extracts, which only differed in their intensity. These metabolite compounds were divided into diterpene lactones, flavonoids, and phenolic acid groups. By using the peak intensities of the 18 compounds identified, the leaves and stem extracts were grouped using PCA.
AB - Andrographis paniculata is known as the king of bitter and it has been widely used as a medicinal plant. The properties of A. paniculata are generally determined by the metabolite composition, which may be influenced by several factors, one of which is the part of the plant extracted. The objectives of this research are to identify putatively the metabolite composition of the stem and the leaves extracts using LC-MS/MS and classify them using PCA. The stem and the leaves samples were separated and extracted using sonication with 70% ethanol. A total of 31 metabolite compounds has been putatively identified. All compounds were identified in the stem and the leaves extracts, which only differed in their intensity. These metabolite compounds were divided into diterpene lactones, flavonoids, and phenolic acid groups. By using the peak intensities of the 18 compounds identified, the leaves and stem extracts were grouped using PCA.
KW - Andrographis paniculata
KW - LC-MS/MS
KW - metabolite profiling
UR - http://www.scopus.com/inward/record.url?scp=85087913379&partnerID=8YFLogxK
U2 - 10.1080/14786419.2020.1789637
DO - 10.1080/14786419.2020.1789637
M3 - Article
C2 - 32657134
AN - SCOPUS:85087913379
SN - 1478-6419
SP - 1
EP - 5
JO - Natural Product Research
JF - Natural Product Research
ER -