Mass induction of tiger barb (Puntius tetrazona) spawning via hormone immersion technique

Rizqi Endah Warni, Ainulyakin Imlani, Suko Ismi, Endang Dewi Masithah, Darmawan Setia Budi

Research output: Contribution to journalArticlepeer-review


The tiger barb (Puntius tetrazona) is a freshwater ornamental fish native to Indonesia, predominantly found in Sumatra and Kalimantan. This research investigated the mass induction of tiger barb propagation through the hormone immersion method using Ovaprim, aiming to optimize its dosage for improved reproductive outcomes. The study systematically evaluated different hormone doses, ranging from 0.0 to 2.0 mL/L, and assessed critical parameters including latency period, total egg production, fertilization rate, hatching rate, and larval survival rate. The results revealed dose-dependent effects, with 1.5 mL/L as the optimal dosage. This concentration resulted in a latency period of 5.50 ± 0.83 h, total egg yield of 448.00 ± 35.18, fertilization rate of 99.04 ± 0.49%, hatching rate of 96.40 ± 1.73%, and larval survival rate of 95.52 ± 1.05%. This study provides valuable insights for the aquaculture industry, offering optimum parameters for the mass propagation induction in tiger barbs using Ovaprim immersion. This study does not only highlight the effectiveness of Ovaprim immersion but also underscores its potential as a powerful tool to improve breeding protocols and address the growing demand for ornamental fish.

Original languageEnglish
JournalEgyptian Journal of Aquatic Research
Publication statusAccepted/In press - 2024


  • Breeding
  • Egg
  • Fish species
  • Hatching rate
  • Ornamental fish
  • Ovaprim


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