TY - JOUR
T1 - Lipopolysaccharide’s cytotoxicity on human umbilical cord mesenchymal stem cells
AU - Kuntjoro, Mefina
AU - Prasetyo, Eric Priyo
AU - Cahyani, Febriastuti
AU - Kamadjaja, Michael Josef Kridanto
AU - Hendrijantini, Nike
AU - Laksono, Harry
AU - Rahmania, Primanda Nur
AU - Ariestania, Vivin
AU - Nugraha, Alexander Patera
AU - Ihsan, Igo Syaiful
AU - Dinaryanti, Aristika
AU - Rantam, Fedik Abdul
N1 - Publisher Copyright:
© 2020, Association of Support to Oral Health Research (APESB). All rights reserved.
PY - 2020
Y1 - 2020
N2 - Objective: To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material and Methods: Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 µg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%. Results: The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 µg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability. Conclusion: LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.
AB - Objective: To show the cytotoxicity of Porphyromonas gingivalis lipopolysaccharide (LPS) on human umbilical cord mesenchymal stem cells (HUCMSCs) to better understand the characteristics for its application in regenerative procedures under periodontopathogen LPS influence. Material and Methods: Ultrapure Porphyromonas gingivalis LPS was used in this study. This research used a frozen stock HUCMSCs, previously confirmed by flow cytometry. The biological characteristics, such as cell morphology, proliferation, and protein expression, were screened. To check the cytotoxicity, HUCMSCs were cultured and divided into two groups, the control group and LPS group with various concentrations from 25 to 0.39 µg/mL. MTT assay was done and the cells were observed and counted. The significance level was set at 5%. Results: The percentage of living HUCMSCs on LPS group were not significantly different among concentrations (p>0.05) from 25 to 0.39 µg/mL, even though there were slight mean decrease between groups, but they were not significant. The duration of 24 hours of exposure of LPS does not significantly lower HUCMSCs viability. Conclusion: LPS does not affect the viability of HUCMSCs. The lower the concentration of LPS, the higher the viability of HUCMSCs.
KW - Cytotoxicity
KW - Lipopolysaccharide
KW - Mesenchymal stem cells
KW - Umbilical cord
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=85092145387&partnerID=8YFLogxK
U2 - 10.1590/pboci.2020.153
DO - 10.1590/pboci.2020.153
M3 - Article
AN - SCOPUS:85092145387
SN - 1519-0501
VL - 20
SP - 1
EP - 7
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
M1 - e0048
ER -