Line probe assay test in new cases of tuberculosis with rifampicin resistance not detected by Xpert MTB/RIF

Soedarsono Soedarsono, Ni Mertaniasih, Helmia Hasan, Tutik Kusmiati, Ariani Permatasari, Deby Kusumaningrum, Whendy Wijaksono

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: In Indonesia, the National guideline for tuberculosis only recommended taking the DST to check INH resistance only for re-Treatment cases of rifampicin-susceptible TB (RS-TB) detected by Xpert MTB/RIF. This study was conducted mainly to evaluate the proportion of isoniazid resistance in new cases of RS-TB according to the Xpert MTB/RIF. Methods: This was an observational descriptive study in RS-TB new patients diagnosed by Xpert MTB/RIF. Sputum samples were examined using first-line LPA and evaluated by culture-based DST. Results of first-line LPA and culture-based DST were compared and presented. Results: Fifty-four new cases of RS-TB (according Xpert MTB/RIF) were enrolled in this study. INH resistance was detected in 4 (7.4%) using FL-LPA and in 5 (9.3%) using culture-based DST. RIF resistance was also found in 1 (1.9%) using FL-LPA and in 2 (3.7%) using culture-based DST. Ethambutol resistance was also detected in 4 (7.4%) using culture-based DST. Conclusion: First-line LPA successfully revealed 4 (7.4%) of Hr-TB in new RS-TB cases detected by the Xpert MTB/RIF. In new cases with RS-TB detected by the Xpert MTB/RIF, FL-LPA can be used as rapid molecular DST to detect RIF and INH resistance followed by culture-based DST to examine other drug resistance.

Original languageEnglish
Pages (from-to)429-434
Number of pages6
JournalInternational Journal of Mycobacteriology
Volume11
Issue number4
DOIs
Publication statusPublished - Oct 2022

Keywords

  • Culture-based drug susceptibility test
  • Drug-resistant tuberculosis
  • First-line line probe assay
  • Isoniazid-resistant tuberculosis
  • Xpert MTB/RIF

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