TY - JOUR
T1 - Line probe assay test in new cases of tuberculosis with rifampicin resistance not detected by Xpert MTB/RIF
AU - Soedarsono, Soedarsono
AU - Mertaniasih, Ni
AU - Hasan, Helmia
AU - Kusmiati, Tutik
AU - Permatasari, Ariani
AU - Kusumaningrum, Deby
AU - Wijaksono, Whendy
N1 - Publisher Copyright:
© 2022 International Journal of Mycobacteriology.
PY - 2022/10
Y1 - 2022/10
N2 - Background: In Indonesia, the National guideline for tuberculosis only recommended taking the DST to check INH resistance only for re-Treatment cases of rifampicin-susceptible TB (RS-TB) detected by Xpert MTB/RIF. This study was conducted mainly to evaluate the proportion of isoniazid resistance in new cases of RS-TB according to the Xpert MTB/RIF. Methods: This was an observational descriptive study in RS-TB new patients diagnosed by Xpert MTB/RIF. Sputum samples were examined using first-line LPA and evaluated by culture-based DST. Results of first-line LPA and culture-based DST were compared and presented. Results: Fifty-four new cases of RS-TB (according Xpert MTB/RIF) were enrolled in this study. INH resistance was detected in 4 (7.4%) using FL-LPA and in 5 (9.3%) using culture-based DST. RIF resistance was also found in 1 (1.9%) using FL-LPA and in 2 (3.7%) using culture-based DST. Ethambutol resistance was also detected in 4 (7.4%) using culture-based DST. Conclusion: First-line LPA successfully revealed 4 (7.4%) of Hr-TB in new RS-TB cases detected by the Xpert MTB/RIF. In new cases with RS-TB detected by the Xpert MTB/RIF, FL-LPA can be used as rapid molecular DST to detect RIF and INH resistance followed by culture-based DST to examine other drug resistance.
AB - Background: In Indonesia, the National guideline for tuberculosis only recommended taking the DST to check INH resistance only for re-Treatment cases of rifampicin-susceptible TB (RS-TB) detected by Xpert MTB/RIF. This study was conducted mainly to evaluate the proportion of isoniazid resistance in new cases of RS-TB according to the Xpert MTB/RIF. Methods: This was an observational descriptive study in RS-TB new patients diagnosed by Xpert MTB/RIF. Sputum samples were examined using first-line LPA and evaluated by culture-based DST. Results of first-line LPA and culture-based DST were compared and presented. Results: Fifty-four new cases of RS-TB (according Xpert MTB/RIF) were enrolled in this study. INH resistance was detected in 4 (7.4%) using FL-LPA and in 5 (9.3%) using culture-based DST. RIF resistance was also found in 1 (1.9%) using FL-LPA and in 2 (3.7%) using culture-based DST. Ethambutol resistance was also detected in 4 (7.4%) using culture-based DST. Conclusion: First-line LPA successfully revealed 4 (7.4%) of Hr-TB in new RS-TB cases detected by the Xpert MTB/RIF. In new cases with RS-TB detected by the Xpert MTB/RIF, FL-LPA can be used as rapid molecular DST to detect RIF and INH resistance followed by culture-based DST to examine other drug resistance.
KW - Culture-based drug susceptibility test
KW - Drug-resistant tuberculosis
KW - First-line line probe assay
KW - Isoniazid-resistant tuberculosis
KW - Xpert MTB/RIF
UR - http://www.scopus.com/inward/record.url?scp=85143994436&partnerID=8YFLogxK
U2 - 10.4103/ijmy.ijmy_176_22
DO - 10.4103/ijmy.ijmy_176_22
M3 - Article
C2 - 36510930
AN - SCOPUS:85143994436
SN - 2212-5531
VL - 11
SP - 429
EP - 434
JO - International Journal of Mycobacteriology
JF - International Journal of Mycobacteriology
IS - 4
ER -
