TY - JOUR
T1 - Ligament/tendon culture under hypoxic conditions
T2 - A systematic review
AU - Rhatomy, Sholahuddin
AU - Utomo, Dwikora Novembri
AU - Prakoeswa, Cita Rosita Sigit
AU - Rantam, Fedik Abdul
AU - Suroto, Heri
AU - Mahyudin, Ferdiansyah
N1 - Publisher Copyright:
© 2021 The Author (s).
PY - 2021
Y1 - 2021
N2 - The hypoxic environment is a substantial factor in maintenance, proliferation, and differentiation of the cell cultures. Low oxygen is known as a potent chondrogenesis stimulus in stem cells that is important for clinical application and engineering of functional cartilage. Hypoxia can potentially induce angiogenesis process by secretion of cytokines. This systematic review goal is to discover the effect of hypoxic condition on tendon/ ligament culture and the best oxygen level of hypoxia for in vitro and in vivo studies. We included 21 articles. A comprehensive review of this database confirms that the hypoxic condition is a substantial factor in the maintenance, proliferation, and differentiation of ligament/tendon cultures. Cell proliferation in the severe hypoxic (oxygen concentration of 1%) group at 24 hours post-cultivation was considered significant, but cell proliferation was markedly inhibited in the severe hypoxic group after 48 h.
AB - The hypoxic environment is a substantial factor in maintenance, proliferation, and differentiation of the cell cultures. Low oxygen is known as a potent chondrogenesis stimulus in stem cells that is important for clinical application and engineering of functional cartilage. Hypoxia can potentially induce angiogenesis process by secretion of cytokines. This systematic review goal is to discover the effect of hypoxic condition on tendon/ ligament culture and the best oxygen level of hypoxia for in vitro and in vivo studies. We included 21 articles. A comprehensive review of this database confirms that the hypoxic condition is a substantial factor in the maintenance, proliferation, and differentiation of ligament/tendon cultures. Cell proliferation in the severe hypoxic (oxygen concentration of 1%) group at 24 hours post-cultivation was considered significant, but cell proliferation was markedly inhibited in the severe hypoxic group after 48 h.
KW - Culture
KW - Hypoxic condition
KW - Ligament
KW - Tendon
UR - http://www.scopus.com/inward/record.url?scp=85117229668&partnerID=8YFLogxK
U2 - 10.34172/APB.2021.069
DO - 10.34172/APB.2021.069
M3 - Review article
AN - SCOPUS:85117229668
SN - 2228-5881
VL - 11
SP - 595
EP - 600
JO - Advanced Pharmaceutical Bulletin
JF - Advanced Pharmaceutical Bulletin
IS - 4
ER -