Levels of Wistar Calcium Serum (Rattus norvegicus) in Human Adipose-derived Mesenchymal Stem Cells (hADMSCs) and Chitosan Scaffold by Osteoinduction Examination

Dian Agustin Wahjuningrum, Setyabudi, Destri Imania, Ria Chusnita, Andi Syahrimayani, Latief Mooduto, Anuj Bhardwaj

Research output: Contribution to journalArticlepeer-review

Abstract

Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process of bone tissue. Bone graft is the gold standard for bone repair. However, the use of bone graft has a limited amount of tissue produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components, stem cells that have self-renewal ability and multilineage differentiation, bioreactor / growth factor, and scaffold. The combination of hADMSCs and chitosan scaffold, is expected to trigger osteoinduction shown by osteogenic markers such as calcium levels. Objectives to prove osteoinduction activity in a combination of Human Adipose-derived Mesenchymal Stem Cell (hADMSCs) and chitosan scaffold using serum calcium levels. There were 12 treatment groups with each group having 4 samples. Groups 1 to 4 were the negative control group at day one, three, seven, and fourteen which maxillary bone drilling only. While groups 5 to 8 were the positive control group at day one, three, seven, and fourteen which were given chitosan scaffold. Groups 9 to 12 were treatment group at day one, three, seven, and fourteen which were given hADMSCs and chitosan scaffold. Blood is collected in each group to check serum calcium levels. There were differences in calcium levels in blood serum in each group.

Original languageEnglish
Pages (from-to)20-23
Number of pages4
JournalJournal of International Dental and Medical Research
Volume16
Issue number1
Publication statusPublished - 2023

Keywords

  • calcium
  • chitosan scaffold
  • human adipose-derived mesenchymal stem cells
  • patient satisfaction
  • tissue engineering

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