TY - JOUR
T1 - Levels of Wistar Calcium Serum (Rattus norvegicus) in Human Adipose-derived Mesenchymal Stem Cells (hADMSCs) and Chitosan Scaffold by Osteoinduction Examination
AU - Wahjuningrum, Dian Agustin
AU - Setyabudi, null
AU - Imania, Destri
AU - Chusnita, Ria
AU - Syahrimayani, Andi
AU - Mooduto, Latief
AU - Bhardwaj, Anuj
N1 - Funding Information:
The authors are grateful to the Indonesian Ministry of Research and Technology and the Faculty of Dental Medicine, Universitas Airlangga, Surabaya for the funding support so this research could be conducted.
Publisher Copyright:
© 2023,Journal of International Dental and Medical Research. All Rights Reserved.
PY - 2023
Y1 - 2023
N2 - Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process of bone tissue. Bone graft is the gold standard for bone repair. However, the use of bone graft has a limited amount of tissue produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components, stem cells that have self-renewal ability and multilineage differentiation, bioreactor / growth factor, and scaffold. The combination of hADMSCs and chitosan scaffold, is expected to trigger osteoinduction shown by osteogenic markers such as calcium levels. Objectives to prove osteoinduction activity in a combination of Human Adipose-derived Mesenchymal Stem Cell (hADMSCs) and chitosan scaffold using serum calcium levels. There were 12 treatment groups with each group having 4 samples. Groups 1 to 4 were the negative control group at day one, three, seven, and fourteen which maxillary bone drilling only. While groups 5 to 8 were the positive control group at day one, three, seven, and fourteen which were given chitosan scaffold. Groups 9 to 12 were treatment group at day one, three, seven, and fourteen which were given hADMSCs and chitosan scaffold. Blood is collected in each group to check serum calcium levels. There were differences in calcium levels in blood serum in each group.
AB - Bone tissue reconstruction with extensive damage is one of the challenges for dentists because its healing process of bone tissue. Bone graft is the gold standard for bone repair. However, the use of bone graft has a limited amount of tissue produced. Tissue engineering is the latest method in terms of bone regeneration. Tissue engineering has three main components, stem cells that have self-renewal ability and multilineage differentiation, bioreactor / growth factor, and scaffold. The combination of hADMSCs and chitosan scaffold, is expected to trigger osteoinduction shown by osteogenic markers such as calcium levels. Objectives to prove osteoinduction activity in a combination of Human Adipose-derived Mesenchymal Stem Cell (hADMSCs) and chitosan scaffold using serum calcium levels. There were 12 treatment groups with each group having 4 samples. Groups 1 to 4 were the negative control group at day one, three, seven, and fourteen which maxillary bone drilling only. While groups 5 to 8 were the positive control group at day one, three, seven, and fourteen which were given chitosan scaffold. Groups 9 to 12 were treatment group at day one, three, seven, and fourteen which were given hADMSCs and chitosan scaffold. Blood is collected in each group to check serum calcium levels. There were differences in calcium levels in blood serum in each group.
KW - calcium
KW - chitosan scaffold
KW - human adipose-derived mesenchymal stem cells
KW - patient satisfaction
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85152642380&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85152642380
SN - 1309-100X
VL - 16
SP - 20
EP - 23
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 1
ER -