TY - JOUR
T1 - Lactobacillus plantarum IS-20506 probiotic restores galectin-4 and myosin-1a expressions in duodenum, jejunum and ileum of lipopolysaccharide-induced rats
AU - Ranuh, Reza Gunadi
AU - Athiyyah, Alpha Fardah
AU - Darma, Andy
AU - Riawan, Wibi
AU - Surono, Ingrid Suryanti
AU - Sandra, Ferry
AU - Sudarmo, Subijanto Marto
N1 - Publisher Copyright:
© 2020 Prodia Education and Research Institute. All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - BACKGROUND: Important border structure proteins and Galectin-4 composition. for normal and Damage intestinal Myosin-1a of brush these are proteins by inflammation may alter digestion, absorption and barrier function. Probiotic has been widely known in maintaining gut health. However, the molecular mechanism of Lactobacillus plantarum IS-2056 probiotic in repairing intestinal brush border is not well defined. Therefore, current study was conducted by investigating the Galectin-4 and Myosin-1a expressions in a rodent model. METHODS: Male Wistar rats were induced with/without lipopolysaccharide (LPS) and treated with/without L. plantarum IS-2056 probiotic. On the seventh day, duodenum, jejunum, and ileum were collected and analyzed with western blot and immunohistochemistry for Galectin-4 and Myosin-1a expressions. RESULTS: Rats administrated with L. plantarum IS-2056 probiotic showed significant increase of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum compared to the control group (p<0.05). While in control group, Galectin-4 level tended to increase in more distal of intestinal segment and Myosin-1a level tended to decrease in more distal intestinal segment. CONCLUSION: L. plantarum IS-20506 probiotic may facilitate the repairment of damaged intestinal brush border as demonstrated by significant restoration of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum of LPS-induced rats.
AB - BACKGROUND: Important border structure proteins and Galectin-4 composition. for normal and Damage intestinal Myosin-1a of brush these are proteins by inflammation may alter digestion, absorption and barrier function. Probiotic has been widely known in maintaining gut health. However, the molecular mechanism of Lactobacillus plantarum IS-2056 probiotic in repairing intestinal brush border is not well defined. Therefore, current study was conducted by investigating the Galectin-4 and Myosin-1a expressions in a rodent model. METHODS: Male Wistar rats were induced with/without lipopolysaccharide (LPS) and treated with/without L. plantarum IS-2056 probiotic. On the seventh day, duodenum, jejunum, and ileum were collected and analyzed with western blot and immunohistochemistry for Galectin-4 and Myosin-1a expressions. RESULTS: Rats administrated with L. plantarum IS-2056 probiotic showed significant increase of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum compared to the control group (p<0.05). While in control group, Galectin-4 level tended to increase in more distal of intestinal segment and Myosin-1a level tended to decrease in more distal intestinal segment. CONCLUSION: L. plantarum IS-20506 probiotic may facilitate the repairment of damaged intestinal brush border as demonstrated by significant restoration of Galectin-4 and Myosin-1a expressions in duodenum, jejunum, and ileum of LPS-induced rats.
KW - Duodenum
KW - Galectin-4
KW - IS-20506
KW - Ileum
KW - Jejunum
KW - Lactobacillus plantarum
KW - Myosin-1a
KW - Probiotic
UR - http://www.scopus.com/inward/record.url?scp=85096918303&partnerID=8YFLogxK
U2 - 10.18585/inabj.v12i3.1098
DO - 10.18585/inabj.v12i3.1098
M3 - Article
AN - SCOPUS:85096918303
SN - 2085-3297
VL - 12
SP - 283
EP - 287
JO - Indonesian Biomedical Journal
JF - Indonesian Biomedical Journal
IS - 3
ER -