TY - JOUR
T1 - Lactiplantibacillus plantarum IS-10506 Enhances Tight Junction Integrity in Bronchial Epithelium
T2 - An Experimental Study
AU - Fetarayani, Deasy
AU - Soegiarto, Gatot
AU - Surono, Ingrid Suryanti
AU - Endaryanto, Anang
AU - Athiyyah, Alpha Fardah
AU - Hernaningsih, Yetti
AU - Fauziah, Dyah
AU - Sudiana, Ketut
AU - Utomo, Budi
AU - Prakoeswa, Cita Rosita Sigit
N1 - Publisher Copyright:
© 2024 The Prodia Education and Research Institute. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (CC-BY-NC) License. All Rights Reserved.
PY - 2024
Y1 - 2024
N2 - BACKGROUND: Airway epithelium constitutes the initial structural defense against inhaled allergens, with its integrity primarily maintained through the formation of tight junctions (TJ) consisting of claudins, occludins, and zonula occludens (ZO). Currently, the effects of probiotics on airway TJ remain unclear. Therefore, this study was conducted to explore the impact of Lactiplantibacillus plantarum IS-10506, a native probiotic from Indonesia, on bronchial epithelial TJ in a mouse model of allergic airway inflammation. METHODS: Twenty-four BALB/c mice were randomly assigned to three groups: negative control, positive control subjected to Dermatophagoides pteryonissinus (Der p) sensitization and challenge, and treatment group exposed to Dcr p and administered L. plantarum IS-10506 since one week before sensitization up to three weeks after. The expression levels of elaudin-18, occludin, and ZO-1 were investigated through immunohistochemistry analysis and quantified based on the immunoreactive score (1RS). RESULTS: The results of this study demonstrated that L. plantarum IS-10506 significantly enhanced the expressions of elaudin-18, occludin, and ZO-1 (p<0.05). CONCLUSION: These findings underscore the probiotic’s role in fortifying the integrity of airway TJ.
AB - BACKGROUND: Airway epithelium constitutes the initial structural defense against inhaled allergens, with its integrity primarily maintained through the formation of tight junctions (TJ) consisting of claudins, occludins, and zonula occludens (ZO). Currently, the effects of probiotics on airway TJ remain unclear. Therefore, this study was conducted to explore the impact of Lactiplantibacillus plantarum IS-10506, a native probiotic from Indonesia, on bronchial epithelial TJ in a mouse model of allergic airway inflammation. METHODS: Twenty-four BALB/c mice were randomly assigned to three groups: negative control, positive control subjected to Dermatophagoides pteryonissinus (Der p) sensitization and challenge, and treatment group exposed to Dcr p and administered L. plantarum IS-10506 since one week before sensitization up to three weeks after. The expression levels of elaudin-18, occludin, and ZO-1 were investigated through immunohistochemistry analysis and quantified based on the immunoreactive score (1RS). RESULTS: The results of this study demonstrated that L. plantarum IS-10506 significantly enhanced the expressions of elaudin-18, occludin, and ZO-1 (p<0.05). CONCLUSION: These findings underscore the probiotic’s role in fortifying the integrity of airway TJ.
KW - allergic inflammation
KW - elaudin
KW - immunology
KW - occludin
KW - probiotic Lacliplantibacillus plantarum
KW - respiratory system
KW - tight junction
KW - zonula occludcns
UR - http://www.scopus.com/inward/record.url?scp=85193529715&partnerID=8YFLogxK
U2 - 10.18585/inabj.v16i2.2995
DO - 10.18585/inabj.v16i2.2995
M3 - Article
AN - SCOPUS:85193529715
SN - 2085-3297
VL - 16
SP - 162
EP - 171
JO - Indonesian Biomedical Journal
JF - Indonesian Biomedical Journal
IS - 2
ER -