TY - JOUR
T1 - Lactic acid’s role in hypertonic sodium lactate solution as a neuroprotector measured from the level of ATP, MCT-1, and necrotic areas in intracerebral hematoma rats model
AU - Hamzah,
AU - Rehatta, Nancy Margarita
AU - Bisri, Tatang
AU - Saleh, Siti Chasnak
AU - Utariani, Arie
N1 - Publisher Copyright:
© 2019, The Indonesian Foundation of Critical Care Medicine. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Objective: To discover the role of hypertonic sodium lactate (HSL) as the energy source, which in turn will act as a neuroprotector, by measuring adenosine triphosphate (ATP) level, monocarboxylate transporter 1 (MCT-1) and the extent of the necrotic areas. Design: This was an experimental study that used randomized post-test only control group design. Setting: Experimental Animal Care Unit Universitas Gadjah Mada. Patient and participant: 32 white mice of Rattus norvegicus. Intervention: After the protocol of this study was approved by the research ethic committee, _ 32 rats were randomly divided into two groups: HSL group (n=16) and NaCl 3% group (n=16) as the control group. Both groups were anesthetized using conversion-dose pentothal. Results: ATP level in HSL group was higher compared to the control group (p=0.031). MCT-1 in HSL group was also higher than the control group (p=0.010). Necrotic areas were less extensive in the HSL group than the control group (p=0.000). Lactate levels at minute 30 (T30) and minute 360 (T360) increased in the HSL group, while increasing in the control group up to T30, then decreased gradually until T360. Conclusion: Exogenous lactate in solution has effect as a neuroprotective of brain in the intracerebral hemorrhage (ICH).
AB - Objective: To discover the role of hypertonic sodium lactate (HSL) as the energy source, which in turn will act as a neuroprotector, by measuring adenosine triphosphate (ATP) level, monocarboxylate transporter 1 (MCT-1) and the extent of the necrotic areas. Design: This was an experimental study that used randomized post-test only control group design. Setting: Experimental Animal Care Unit Universitas Gadjah Mada. Patient and participant: 32 white mice of Rattus norvegicus. Intervention: After the protocol of this study was approved by the research ethic committee, _ 32 rats were randomly divided into two groups: HSL group (n=16) and NaCl 3% group (n=16) as the control group. Both groups were anesthetized using conversion-dose pentothal. Results: ATP level in HSL group was higher compared to the control group (p=0.031). MCT-1 in HSL group was also higher than the control group (p=0.010). Necrotic areas were less extensive in the HSL group than the control group (p=0.000). Lactate levels at minute 30 (T30) and minute 360 (T360) increased in the HSL group, while increasing in the control group up to T30, then decreased gradually until T360. Conclusion: Exogenous lactate in solution has effect as a neuroprotective of brain in the intracerebral hemorrhage (ICH).
KW - ATP
KW - Extent of necrotic areas
KW - Hypertonic sodium lactate
KW - Intracerebral hematoma
KW - Neuroprotector
UR - http://www.scopus.com/inward/record.url?scp=85079457567&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85079457567
SN - 1410-7767
VL - 22
SP - 98
EP - 108
JO - Critical Care and Shock
JF - Critical Care and Shock
IS - 2
ER -