Karakterisasi Molekuler Protein Hemagglutinin-Neuraminidase Virus Newcastle Disease (ND) di Surabaya Tahun 2003 dan 2016

Translated title of the contribution: Molecular characterization of hemagglutinin-neuraminidase protein virus newcastle disease (Nd) in surabaya during 2003 and 2016

Innah Wulandari, Jola Rahmahani, Indah Rahmawati, Nurvita Putih, Aisyah Azahro, Kusnoto Kusnoto, Rahayu Ernawati, Fedik Abdul Rantam

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This study aimed to determine the mutation of amino acid, nucleotide homology, phylogenetic tree, epitope prediction of Hemagglutinin-Neuraminidase protein Newcastle Disease (ND) Virus isolated from traditional market around Surabaya. Samples were from 37 chicken with cloacal swab and one positive samples for control (LaSota). Samples were inoculated on embyonate chicken eggs and identified with HA test confirmed with HI test. Positive samples processed by PCR using forward and reverse primer with 503 bp RNA target. The PCR result then analyzed with sequencing. Result of sequencing analysis showed that theres similiarity between samples amino acid and vaccine isolate its effect the percentage of nucleotide homology and phylogenetic relation between isolate. Epitope NGAANNSGWGAPIHDPDYIGG have high immunogenic value at all of isolate which good as vaccine candidate.

Translated title of the contributionMolecular characterization of hemagglutinin-neuraminidase protein virus newcastle disease (Nd) in surabaya during 2003 and 2016
Original languageIndonesian
Pages (from-to)231-242
Number of pages12
JournalJurnal Medik Veteriner
Volume4
Issue number2
DOIs
Publication statusPublished - Oct 2021

Keywords

  • Epitope
  • Hnprotein
  • Newcastle Disease
  • Nucleotide homology
  • Phylogenetic tree

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