Isolation of lipolytic bacteria from domestic waste compost and its application to biodiesel production

Purkan Purkan, Indah Tri Lestari, Rizky Arissirajudin, Retno Rahayu Puspita Ningsih, Wiwin Apriyani, Hamidah Nurlaila, Sri Sumarsih, Sofijan Hadi, Wiwin Retnowati, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


The exploration of lipolytic bacteria presents its challenges in being able to supply lipase as a catalyst in the production of biodiesel. The compost is chosen to get the bacteria because of the lipid and fatty acids component contained in it. The research was developed to isolate the lipase-producing bacteria and determine the enzyme ability in bioconversion of lipid to biodiesel. The bacterial screening was conducted in Luria Bertani media with additional olive oil 1% (v/v) as an inducer. The colonies surrounded by bright areas were separated from others because they indicated having lipolytic activity. Identification of selected isolate was carried out according to the 16srRNA fragment DNA analysis. The fragment showed a score of nucleotide identity as 98% with the Proteus sp. The isolated bacteria grow well in LB media forming an exponential phase from 2 to 11 h, and have a double-time of cell growth at the 4 h. It produced lipase optimally at the 19 hours with its activity of 1.771 U/mL. The enzyme showed optimum activity at 45oC and pH 8. The lipase showed high activity in t-butanol then followed by sequentially in a mixed solvent (methanol and tbutanol), and a single solvent of isopropanol, methanol, and n-hexane. It exhibited good performance for the biodiesel production from microalgal oil, yielded a total fatty acid methyl esters (FAME) as 2.75% (v/v). The fatty acid composition of the methyl esters was composed of 9 octadecanoic acid and hexadecanoic acid.

Original languageEnglish
Pages (from-to)2074-2084
Number of pages11
JournalRasayan Journal of Chemistry
Issue number4
Publication statusPublished - 2020


  • 16srRNA
  • Biodiesel
  • Lipase
  • Lipolytic Bacteria


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