TY - JOUR
T1 - Isolation of glucosamine hcl from scylla paramamosain and determination by HPLC
AU - Putri, Astrid Kusuma
AU - Kartosentono, Sugijanto
AU - Sugijanto, Noor Erma Nasution
N1 - Funding Information:
We are indebted to the Kementerian Pendidikan Tinggi dan Ristek Republik Indonesia for financial support by Universitas Airlangga, Kontrak Penelitian NO: 1487/UN3.1.5/KU/2017.
Publisher Copyright:
© 2019 Penerbit UTM Press.
PY - 2019/9
Y1 - 2019/9
N2 - Scylla paramamosain is one of crustaceans that has economic value in Indonesia. About 40-50% of the total weight of crustaceans goes as waste after being processed to be human food. The Crustacean waste needs to be reduced in order to minimize the negative impact on the environment. Whereas products derived from crustacean shell also has medical value (e.g. Chitosan, Glucosamine HCl). Glucosamine is an amino monosaccharide acting as a substrate for the production of aggrecan and proteoglycans and thus have therapeutic activity in osteoarthritis. The present study has been aimed to prepare glucosamine hydrochloride (GluHCl) from S. paramamosain waste by acid hydrolysis in four treatment groups and their quantitation by high-performance liquid chromatography (HPLC). The best condition from this experiment is shell deproteination with 3% NaOH solution (w/v 1:6), at 85ºC, for 30 minutes, demineralisation with 1N HCl (w/v 1:10), at 75ºC, for 1.5 hours, depigmentation with NaClO 0.38%, at room temperature, for 1 hour, and hydrolysis with conc. HCl (w/v 1:20), at 85ºC, for 18 minutes, resulted in the yield of 6.15 ± 0.62% and quantitation by HPLC analysis of obtained glucosamine hydrochloride was 98.48 ± 0.74%. Further, this study describes the recycling of S. paramamosain waste to a value-added product which is having potential applications in the field of food and medicine.
AB - Scylla paramamosain is one of crustaceans that has economic value in Indonesia. About 40-50% of the total weight of crustaceans goes as waste after being processed to be human food. The Crustacean waste needs to be reduced in order to minimize the negative impact on the environment. Whereas products derived from crustacean shell also has medical value (e.g. Chitosan, Glucosamine HCl). Glucosamine is an amino monosaccharide acting as a substrate for the production of aggrecan and proteoglycans and thus have therapeutic activity in osteoarthritis. The present study has been aimed to prepare glucosamine hydrochloride (GluHCl) from S. paramamosain waste by acid hydrolysis in four treatment groups and their quantitation by high-performance liquid chromatography (HPLC). The best condition from this experiment is shell deproteination with 3% NaOH solution (w/v 1:6), at 85ºC, for 30 minutes, demineralisation with 1N HCl (w/v 1:10), at 75ºC, for 1.5 hours, depigmentation with NaClO 0.38%, at room temperature, for 1 hour, and hydrolysis with conc. HCl (w/v 1:20), at 85ºC, for 18 minutes, resulted in the yield of 6.15 ± 0.62% and quantitation by HPLC analysis of obtained glucosamine hydrochloride was 98.48 ± 0.74%. Further, this study describes the recycling of S. paramamosain waste to a value-added product which is having potential applications in the field of food and medicine.
KW - Chitin
KW - Chitosan
KW - Crustaceans
KW - Glucosamine hydrochloride
KW - Scylla paramamosain
UR - http://www.scopus.com/inward/record.url?scp=85073340221&partnerID=8YFLogxK
U2 - 10.11113/jt.v81.13416
DO - 10.11113/jt.v81.13416
M3 - Article
AN - SCOPUS:85073340221
SN - 0127-9696
VL - 81
SP - 1
EP - 8
JO - Jurnal Teknologi (Sciences and Engineering)
JF - Jurnal Teknologi (Sciences and Engineering)
IS - 5
ER -
