TY - GEN
T1 - Isolation and screening of potential proteolytic and amylolytic microbes from Wonorejo Mangrove forest soil, Surabaya, Indonesia
AU - Khiftiyah, Ana Mariatul
AU - Hanifah, Nabela Nur
AU - Bachruddin, Muhammad
AU - Sholichah, Mar'Atus
AU - Istiqomah, Siti
AU - Dewi, Selva Rosyta
AU - Rahayu, Tri
AU - Prasetya, Indra Adi Wira
AU - Marjayandari, Lisa
AU - Aini, Nurul
AU - Tsana, Izdihar
AU - Triwahyuni, Desi
AU - Fatimah,
AU - Ni'Matuzahroh,
N1 - Publisher Copyright:
© 2018 Author(s).
PY - 2018/10/10
Y1 - 2018/10/10
N2 - The aims of this research were to isolate and to identify the microbes that have potential amylolytic and proteolytic activities. Microbial isolation was performed on mangrove soil sample from Wonorejo, Surabaya, by plating samples on Nutrient Agar (NA), Potato Dextrose Agar (PDA), Nitrogen free bromothymol blue (Nfb), Carboxymethylcellulose (CMC), and Pikovskaya agar media. Microbial isolates obtained were grown on Bushnell Haas agar enriched with starch or milk to test production of amylase and protease enzymes. The results revealed three kinds of bacteria and six kinds of moulds that were purified and characterised. The two kinds of bacteria had NA1 and CMC1 isolate codes and the two kinds of moulds were PDA20131 and PIKOV1513, which could grow on PDA and Pikovskaya media, and were used to test their amylolytic and proteolytic activities. Results of proteolytic tests revealed that there were two microbial isolates that could produce proteases, i.e. PDA20131 and a Gram-negative rod bacteria (NA1). The isolates that produced amylases were PIKOV1513 and a Gram-negative rod bacteria capable of growing on the CMC media (CMC1).
AB - The aims of this research were to isolate and to identify the microbes that have potential amylolytic and proteolytic activities. Microbial isolation was performed on mangrove soil sample from Wonorejo, Surabaya, by plating samples on Nutrient Agar (NA), Potato Dextrose Agar (PDA), Nitrogen free bromothymol blue (Nfb), Carboxymethylcellulose (CMC), and Pikovskaya agar media. Microbial isolates obtained were grown on Bushnell Haas agar enriched with starch or milk to test production of amylase and protease enzymes. The results revealed three kinds of bacteria and six kinds of moulds that were purified and characterised. The two kinds of bacteria had NA1 and CMC1 isolate codes and the two kinds of moulds were PDA20131 and PIKOV1513, which could grow on PDA and Pikovskaya media, and were used to test their amylolytic and proteolytic activities. Results of proteolytic tests revealed that there were two microbial isolates that could produce proteases, i.e. PDA20131 and a Gram-negative rod bacteria (NA1). The isolates that produced amylases were PIKOV1513 and a Gram-negative rod bacteria capable of growing on the CMC media (CMC1).
KW - Amylolytic
KW - growth
KW - microbes
KW - proteolytic
UR - http://www.scopus.com/inward/record.url?scp=85055509830&partnerID=8YFLogxK
U2 - 10.1063/1.5061884
DO - 10.1063/1.5061884
M3 - Conference contribution
AN - SCOPUS:85055509830
T3 - AIP Conference Proceedings
BT - 9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University
A2 - Huyop, Fahrul Zaman Bin
A2 - Siswanto, Dian
A2 - Mastuti, Retno
A2 - Treesubsuntorn, Chairat
PB - American Institute of Physics Inc.
T2 - 9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018
Y2 - 7 March 2018 through 8 March 2018
ER -