Isolation and screening of potential proteolytic and amylolytic microbes from Wonorejo Mangrove forest soil, Surabaya, Indonesia

Ana Mariatul Khiftiyah, Nabela Nur Hanifah, Muhammad Bachruddin, Mar'Atus Sholichah, Siti Istiqomah, Selva Rosyta Dewi, Tri Rahayu, Indra Adi Wira Prasetya, Lisa Marjayandari, Nurul Aini, Izdihar Tsana, Desi Triwahyuni, Fatimah, Ni'Matuzahroh

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Citations (Scopus)

Abstract

The aims of this research were to isolate and to identify the microbes that have potential amylolytic and proteolytic activities. Microbial isolation was performed on mangrove soil sample from Wonorejo, Surabaya, by plating samples on Nutrient Agar (NA), Potato Dextrose Agar (PDA), Nitrogen free bromothymol blue (Nfb), Carboxymethylcellulose (CMC), and Pikovskaya agar media. Microbial isolates obtained were grown on Bushnell Haas agar enriched with starch or milk to test production of amylase and protease enzymes. The results revealed three kinds of bacteria and six kinds of moulds that were purified and characterised. The two kinds of bacteria had NA1 and CMC1 isolate codes and the two kinds of moulds were PDA20131 and PIKOV1513, which could grow on PDA and Pikovskaya media, and were used to test their amylolytic and proteolytic activities. Results of proteolytic tests revealed that there were two microbial isolates that could produce proteases, i.e. PDA20131 and a Gram-negative rod bacteria (NA1). The isolates that produced amylases were PIKOV1513 and a Gram-negative rod bacteria capable of growing on the CMC media (CMC1).

Original languageEnglish
Title of host publication9th International Conference on Global Resource Conservation, ICGRC and AJI from Ritsumeikan University
EditorsFahrul Zaman Bin Huyop, Dian Siswanto, Retno Mastuti, Chairat Treesubsuntorn
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417373
DOIs
Publication statusPublished - 10 Oct 2018
Event9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018 - Malang City - East Java, Indonesia
Duration: 7 Mar 20188 Mar 2018

Publication series

NameAIP Conference Proceedings
Volume2019
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference9th International Conference on Global Resource Conservation and AJI from Ritsumeikan University, ICGRC 2018
Country/TerritoryIndonesia
CityMalang City - East Java
Period7/03/188/03/18

Keywords

  • Amylolytic
  • growth
  • microbes
  • proteolytic

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