TY - JOUR
T1 - Isolation and characterization of skin derived mesenchymal stem cell (SMSCs) from New Zealand rabbit, Oryctolagus Cuniculus
T2 - A in vitro study
AU - Dinaryanti, Aristika
AU - Karsari, Deya
AU - Ertanti, Nora
AU - Ihsan, Igo Syaiful
AU - Ariyanti, Aida
AU - Rantam, Fedik Abdul
AU - Aulanni'am, Aulani
AU - Purwati,
N1 - Publisher Copyright:
© 2019 Connect Journal.
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Mesenchymal stem cells (MSCs) exhibit high proliferation and self-renewal capabilities and are critical for tissue repair and regeneration. Furthermore, they can be isolated from a variety of tissues and have many potential applications in the clinical setting. In this study, Skin Derived Mesenchymal Stem Cells (SMSCs) were isolated from a New Zealand rabbit and analyzed for the skin sampling area, separation method, culture conditions, primary and passage culture times, and cell surface markers. The result shows that SMSCs have surface markers of mesenchymal stem cells by immunofluorescence of CD105, CD90, CD73, CD44, and CD200 and the CD105, CD90, and CD73 positive markers were also confirmed with Flow cytometry. Furthermore, negative markers of CD45 and CD34 did not show in immunofluorescence but CD45, CD34, and CD133 were confirmed with Flow cytometry.
AB - Mesenchymal stem cells (MSCs) exhibit high proliferation and self-renewal capabilities and are critical for tissue repair and regeneration. Furthermore, they can be isolated from a variety of tissues and have many potential applications in the clinical setting. In this study, Skin Derived Mesenchymal Stem Cells (SMSCs) were isolated from a New Zealand rabbit and analyzed for the skin sampling area, separation method, culture conditions, primary and passage culture times, and cell surface markers. The result shows that SMSCs have surface markers of mesenchymal stem cells by immunofluorescence of CD105, CD90, CD73, CD44, and CD200 and the CD105, CD90, and CD73 positive markers were also confirmed with Flow cytometry. Furthermore, negative markers of CD45 and CD34 did not show in immunofluorescence but CD45, CD34, and CD133 were confirmed with Flow cytometry.
KW - Flow cytometry
KW - Immunofluorescence
KW - Mesenchymal stem cells
KW - SMSCs
UR - http://www.scopus.com/inward/record.url?scp=85079432054&partnerID=8YFLogxK
U2 - 10.35124/bca.2019.19.S2.4797
DO - 10.35124/bca.2019.19.S2.4797
M3 - Article
AN - SCOPUS:85079432054
SN - 0972-5075
SP - 4797
EP - 4801
JO - Biochemical and Cellular Archives
JF - Biochemical and Cellular Archives
ER -