Isolasi dan Identifikasi Protein Spike (Protein-S) pada Isolat Lapangan Infectious Bronchitis Virus

Translated title of the contribution: Isolation and Identification of Protein Spike (Protein-S) in Field Isolates of Infectious Bronchitis Virus

Jola Rahmahani, Dewanggi Kristi Sasmi Pradhita, Nanik Sianita Widjaja, Suwarno

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Infectious Bronchitis (IB) is disease causes great economic impact across nations. Spike protein (protein-S) is one of structural protein of Infectious Bronchitis Virus (IBV) located on enveloped of the virion. Molecular characterization of IBV could be conducted use Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to reveal the protein weight then confirmed through Immuno-Blotting assay. These methods were conducted to know the molecule weight of field isolate compared to seed vaccine. The results of this study indicate the weight of protein-S of field isolate I-147/11 were 168.21 kDa which was same to protein-S of seed vaccine Massachusset.

Translated title of the contributionIsolation and Identification of Protein Spike (Protein-S) in Field Isolates of Infectious Bronchitis Virus
Original languageIndonesian
Pages (from-to)104-109
Number of pages6
JournalJurnal Medik Veteriner
Volume4
Issue number1
DOIs
Publication statusPublished - Apr 2021

Keywords

  • Infectious Bronchitis Virus
  • Spike Glycoprotein
  • field isolate
  • vaccine

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