Abstract
Infectious Bronchitis (IB) is disease causes great economic impact across nations. Spike protein (protein-S) is one of structural protein of Infectious Bronchitis Virus (IBV) located on enveloped of the virion. Molecular characterization of IBV could be conducted use Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to reveal the protein weight then confirmed through Immuno-Blotting assay. These methods were conducted to know the molecule weight of field isolate compared to seed vaccine. The results of this study indicate the weight of protein-S of field isolate I-147/11 were 168.21 kDa which was same to protein-S of seed vaccine Massachusset.
Translated title of the contribution | Isolation and Identification of Protein Spike (Protein-S) in Field Isolates of Infectious Bronchitis Virus |
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Original language | Indonesian |
Pages (from-to) | 104-109 |
Number of pages | 6 |
Journal | Jurnal Medik Veteriner |
Volume | 4 |
Issue number | 1 |
DOIs | |
Publication status | Published - Apr 2021 |
Keywords
- Infectious Bronchitis Virus
- Spike Glycoprotein
- field isolate
- vaccine