Isolasi dan Identifikasi Bakteri Escherichia coli Kontaminan Pada Daging Ayam Broiler Di Rumah Potong Ayam Kabupaten Lamongan

Translated title of the contribution: ISOLATION AND IDENTIFICATION OF Escherichia coli AS BACTERIAL CONTAMINATION IN BROILER CHICKEN MEAT IN POULTRY SLAUGHTERHOUSE LAMONGAN DISTRICT

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14 Citations (Scopus)

Abstract

This study aimed to isolate, and identify, Escherichia coli from Poultry Slaughterhouse in Lamongan District. The sample used in this study was broiler chicken meat taken in July 2018 from Poultry Slaughterhouse in Lamongan District. In this study, 25 grams of Musculus pectoralis from chicken meat used as a sample and amount of samples was 26 samples of chicken meat taken from Poultry Slaughterhouse in Lamongan District. The samples were primary isolated to Eosine Methylene Blue Agar (EMBA) as medium to grow bacteria, then secondary isolation was carried out on Eosine Methylene Blue Agar (EMBA) which aims to seperate the Escherichia coli suspected with other Coliform bacteria. Identification of Escherichia coli using biochemical tests, namely IMViC Test which is useful for identifying Enterobacteriaceae bacteria consisting of Indol Test, Methyl Red Test (MR), Voges Proskauer Test (VP) and Citrate Test. The results of the research conducted showed that there were 3 positive samples of Escherichia coli from 26 samples used in this study. The conclusions obtained from the results of the study were, were as many as 3 positive samples of Escherichia coli while the other 23 samples were negative to Escherichia coli with the percentage of positive samples of Escherichia coli was 11.5% while the percentage of negative samples of Escherichia coli was 88.5%.

Translated title of the contributionISOLATION AND IDENTIFICATION OF Escherichia coli AS BACTERIAL CONTAMINATION IN BROILER CHICKEN MEAT IN POULTRY SLAUGHTERHOUSE LAMONGAN DISTRICT
Original languageIndonesian
Pages (from-to)66-71
Number of pages6
JournalJurnal Medik Veteriner
Volume2
Issue number1
DOIs
Publication statusPublished - Apr 2019

Keywords

  • chicken meat
  • Escherichia coli
  • Lamongan District
  • Poultry Slaughterhouse

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