This study aimed to investigate the involvement of the NLRP3 in activating the immune response after resin monomer 2-hydroxyethyl methacrylate (HEMA) exposure in dentine-pulp complex. The research samples were 28 male Sprague Daley rats in randomized post-test only control groups. The rats were randomly divided into 4 groups, with 7 rats per group. Group 1, the control group was treated with glass ionomer cement restoration (Fuji IX LC) without HEMA application after the lower molar was drilled using a low speed round bur. Group 2-4, the experimental groups were treated with HEMA liquid (Sigma Aldrich) and sealed with glass ionomer cement restoration after the lower molar was drilled using a low speed round bur, and the teeth in each group were extracted after 24, 48 and 72 hours accordingly. The teeth were soaked into EDTA for 8 weeks, then cut 5milimicron by microtome then made the paraffin block. Immunohistochemistry staining was smeared using anti NLRP3 antibody monoclonal. The samples were then statistically analyzed using ANOVA and Tukey HSD. The NLRP3 expression of odontoblast reached the highest number at 48 hours of experimental time. NLRP3 has a role in activating the immune response after resin HEMA exposure in dentine-pulp complex.
|Number of pages||5|
|Journal||Biochemical and Cellular Archives|
|Publication status||Published - Oct 2020|
- dentine-pulp complex
- resin HEMA