TY - JOUR
T1 - Intravaginal delivery of reverse micellar epigallocatechin loaded in ĸ-carrageenan and HPMC K100M-based gel
AU - Rosita, Noorma
AU - Hariyadi, Dewi Melani
AU - Amelia, Cordellia Calista
AU - MAMURRİE, Alyajilan Madani
AU - Miatmoko, Andang
N1 - Publisher Copyright:
© 2022 Marmara University Press.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - A gel with mucoadhesive properties and a controlled release profile is a suitable dosage form for reverse micellar EGCG delivery. In this study, ĸ-Carrageenan and HPMC K100M were used as the gel components at a weight ratio of 1:1.5, respectively, for loading native and reverse micellar EGCG. The characteristics of the gel were determined based on pH, swelling index, disintegration time, hardness, and entrapment efficiency. The in vitro EGCG release rate was further determined for EGCG levels. Moreover, in vivo cervical penetration studies of rhodamine-labeled EGCG gels in mice at two and six hours after intravaginal administrations were conducted. The results showed that the pH and hardness characteristics of the gels for each formula did not differ significantly, while the gel-loaded reverse micellar EGCG had a higher swelling index than that of native EGCG gels. In addition, the rate of release and cervical penetration of rhodamine-labeled reverse micellar EGCG loaded in gels was higher than those of rhodamine-labeled native EGCG gels. Therefore, it can be concluded that loading reverse micelles EGCG into gels prepared with ĸ-Carrageenan and HPMC K100M successfully controlled the release rate and improved cervical penetration, thereby enabling its potential use in cervical cancer treatment.
AB - A gel with mucoadhesive properties and a controlled release profile is a suitable dosage form for reverse micellar EGCG delivery. In this study, ĸ-Carrageenan and HPMC K100M were used as the gel components at a weight ratio of 1:1.5, respectively, for loading native and reverse micellar EGCG. The characteristics of the gel were determined based on pH, swelling index, disintegration time, hardness, and entrapment efficiency. The in vitro EGCG release rate was further determined for EGCG levels. Moreover, in vivo cervical penetration studies of rhodamine-labeled EGCG gels in mice at two and six hours after intravaginal administrations were conducted. The results showed that the pH and hardness characteristics of the gels for each formula did not differ significantly, while the gel-loaded reverse micellar EGCG had a higher swelling index than that of native EGCG gels. In addition, the rate of release and cervical penetration of rhodamine-labeled reverse micellar EGCG loaded in gels was higher than those of rhodamine-labeled native EGCG gels. Therefore, it can be concluded that loading reverse micelles EGCG into gels prepared with ĸ-Carrageenan and HPMC K100M successfully controlled the release rate and improved cervical penetration, thereby enabling its potential use in cervical cancer treatment.
KW - Epigallocatechin gallate
KW - cancer
KW - cervical penetration
KW - hydroxypropyl methylcellulose
KW - reverse micelle
KW - κ-carrageenan
UR - http://www.scopus.com/inward/record.url?scp=85134589287&partnerID=8YFLogxK
U2 - 10.29228/jrp.184
DO - 10.29228/jrp.184
M3 - Article
AN - SCOPUS:85134589287
SN - 1309-0801
VL - 26
SP - 849
EP - 858
JO - Journal of Research in Pharmacy
JF - Journal of Research in Pharmacy
IS - 4
ER -