Intradermal Irritation Assessment of Ethyl Acetate Extract of Sarang Semut (Myrmecordia pendans) on Rat Skin: An In-Depth In Vivo Study

Lusi Epsilawati, Azhari, Mieke Hermiawati Satari, Bambang Pontjo Priosoeryanto, Tri Isyani Tungga Dewi, Merry Annisa Damayanti, Aga Satria Nurrachman

Research output: Contribution to journalArticlepeer-review


Bone defects, arising from conditions such as periodontitis and fractures, present formidable challenges due to their complex and intricate healing processes, often leading to failures or delays. Consequently, there is a pressing need for innovative strategies to expedite this healing trajectory. One promising avenue lies in the exploration of medicinal plants indigenous to specific regions, such as sarang semut (Myrmecodia pendans) from Indonesia. This study conducted sensitivity and irritation tests on male Wistar rats, segregated into test and control groups. The group received 1 cc of physiological NaCl or 1 cc of the test material, namely, ethyl acetate extract of sarang semut containing flavonoids. Skin changes, including edema, erythema, and edema area measurements, were monitored over a span of 24 hours. Remarkably, both groups exhibited no signs of erythema. Regarding edema, the control group scored zero at 12 hours, while the test group reached this at 24 hours, indicating slight irritation (PII index < 2 mm). Crucially, statistical analyses revealed no significant differences in edema diameter between the groups. This evidence underscores that the ethyl acetate extract of sarang semut, enriched with flavonoids, does not induce irritation. Hence, it emerges as a promising avenue for further exploration in subsequent scholarly investigations, offering potential solutions for challenging bone defect scenarios.

Original languageEnglish
Pages (from-to)1610-1615
Number of pages6
JournalJournal of International Dental and Medical Research
Issue number4
Publication statusPublished - 2023


  • Bone Defect
  • Irritating skin test
  • Myrmecodia pendans
  • Sarang semut
  • Sensitivity test


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