Influence of Explant Position on Growth of Talinum paniculatum Gaertn. Adventitious Root in Solid Medium and Enhance Production Biomass in Balloon Type Bubble Bioreactor

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Abstract

Talinum paniculatum Gaertn. is one of traditional medicinal plant in Indonesia as an aphrodisiac. This plant has various compounds which is accumulated in roots. In vitro culture of this plant can enhance production of adventitious roots. The aim of this research was to know the influence of explants position on growth of T. paniculatum Gaertn. adventitious root in MS solid medium and enhance the production of biomass in balloon type bubble bioreactor. Explants from leaf were cultured at abaxial and adaxial positions in solid MS medium supplemented with IBA 2 mgL-1. Adventitious roots were cultured in bioreactor with various treatments (without IBA, supplemented with IBA 2 mgL-1 and supplemented with IBA 2 mgL-1 + buffer NaHCO3). Result showed that the main growth of abaxial root was higher than adaxial, however, the total of adaxial root branch was higher than abaxial. The highest biomass production of adventitious root cultured was achieved by MS medium supplemented with IBA 2 mgL-1 + buffer NaHCO3. This treatment has produced fresh biomass two fold of initial inoculum.

Original languageEnglish
Article number012023
JournalIOP Conference Series: Earth and Environmental Science
Volume58
Issue number1
DOIs
Publication statusPublished - 4 Apr 2017
Event3rd International Seminar on Sciences: Sciences on Precision and Sustainable Agriculture, ISS 2016 - Bogor, Indonesia
Duration: 4 Nov 2016 → …

Keywords

  • Abaxial
  • NaHCO Talinum paniculatum
  • adaxial
  • biomass
  • bioreactor

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