In vivo neuroprotective activity of erythropoietin-alginate microspheres at different polymer concentrations

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Aim: The aim of this study was to evaluate in vivo neuroprotectant activity of erythropoietin-alginate microspheres with different concentrations to modify release to reduce the administration frequency of erythropoietin. Materials and Methods: Sodium alginate and CaCl 2 were used to produce microspheres using aerosolization technique and erythropoietin as model. Balb/c-strain mice (Mus musculus) were used to study in vivo activity in terms of locomotor activity and glutathione peroxidase (GSHPx) activity as well as percentage of reticulocytes. Results and Discussion: Erythropoietin-alginate microspheres demonstrated neuroprotectant activity such as locomotor activity and GSHPx activity compared to erythropoietin alone and blank microspheres. Increased polymer concentrations (1–3%), however, did not have a significant effect on changes of the effectiveness of the activity of microspheres. Interestingly, these results also showed that erythropoietin-alginate microspheres which contain only 5000 units produced a high percentage of reticulocytes. Conclusion: The study indicates that erythropoietin-alginate microspheres showed the potential activity of erythropoietin-alginate microspheres although in low concentration. Increased concentrations of polymers showed similar effects in locomotors activity and GSHPx enzyme activity as parameters of neuroprotectant activity.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalAsian Journal of Pharmaceutics
Issue number4
Publication statusPublished - 1 Oct 2018


  • Alginate microspheres
  • Erythropoietin
  • Glutathione peroxidase
  • In vivo activity
  • Neuroprotectant


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