@article{c2ada75db97644d9b681dd0dda8a0af5,
title = "In vivo effectivity of collagen extract from Clarias gariepinus (burchell, 1822) {\textquoteleft}sangkuriang{\textquoteright} on formation of malondialdehyde and macrophages in burn healing",
abstract = "Topical collagen on skin wound gift moist state that could stimulate reepithelization, proliferation, and epithelial cell migration to conduct growth factor activity on the wound area. One of the sources of collagen is Sangkuriang catfish [Clarias gariepinus (Burchell, 1822) {\textquoteleft}Sangkuriang{\textquoteright}] skin that had low immunoreactivity in the human body. This study aimed to determine the influence of topical treatment with Sangkuriang catfish skin extract on malondialdehyde serum and macrophage total on burn injury. Collagen of Sangkuriang catfish skin was extracted by HCl 2 % for 48 h and neutralized by NaOH 1 M. Sangkuriang catfish skin extract contained amino acid of collagen that could repair and stimulate skin cell. This study was an experimental study that used 24 male Rattus norvegicus strain Wistar were divided into K3, P3, K10, and P10 groups. K group was treated with aquadest topical as a burn healing treatment for 3 d (K3) and 10 d (K10). The P group was treated with topical collagen extract as a burn healing treatment for 3 d (P3) and 10 d (P10). The results showed that treatment by collagen from Sangkuriang catfish skin could not decrease MDA serum on 3rd d and 10 d because of P > 0.05 (P = 0.713). Furthermore, it could not decrease macrophage total on 3rd d (P = 0.427) but significantly decrease to 10 d for K10 and P10. Collagen topical from Sangkuriang catfish could accelerate burn to heal by decreasing macrophages total on 10th d due to P < 0.05 (P = 0.000).",
keywords = "Burn injury, Catfish, Collagen extract, Skin, Wound healing",
author = "Ary Andini and Retno Handajani and Soetjipto Koesnowidagdo and Ichsan, {Miranda Zawazi} and Luciano Pighinelli",
note = "Funding Information: Table 3. p-value of post hoc test on macrophages total of R. norvegicus burns Table Tre3a.tpm-vealnt ue groofuppost hoc test oK3n macrophages toP3tal of R. norK10vegicus burns TreatmeK3nt group K3 0.P4273 K100.000 K3P30.4270.0.000000 K1P30 0.000 K1P100 * Post HoPc10LSD test (p-value < 0.05) * Post Hoc LSD test (p-value < 0.05) 4. CONCLUSIONS 4. CONCLUSIONS th P3 group was lower than K3 group, also on 10 Codll asgheonw teodp icthaal t frdoemscriSpatnivgekluyr iamnagcrcoapthfiasghes [Ctolatarli as Collagen topical from Sangkuriang catfish [Clarias garoife pPi1n0u sg r(oBuupr cwhealsl ,l o1w82e2r )th{\textquoteleft}aSna nKg1k0u rgiarnogu{\textquoteright}p] acnodu lhda dn ot gariepinus (Burchell, 1822) {\textquoteleft}Sangkuriang{\textquoteright}] could nrdot decrease malondialdehyde levels significantly on 3rd d decrease malondialdehyde levels significantly on 3 d and 10th d, also macrophage total on 3rd d. But could adecndm r1eaa0csre odp,hm aaacglseorsophag tmotaaclr te ohpahttot awgaloeu tnsoditga hnilefoainlcain3ngt lwyda.s on 10th Bgouitncgo thtuol d d. quickly ends until the proliferation phase came and Therefore, Collagen topical from Sangkuriang catfish stimulate matrix extracellular (ECM) formation. could accelerate burn healing by decreasing could accelerate burnthhealing by decreasing macrophages total on 10th d. Collagen extract from macrophages total on 10 d. Collagen extract from Sangkuriang catfish is expected to be developed in Sangkuriang catfish is expected to be developed in further research as an effective treatment for the burns further research as an effective treatment for the burns healing process. healing process. 5. ACKNOWLEDGEMENTS 5. ACKNOWLEDGEMENTS Partially of research publication was supported by the Institute of Research and the Community Empowerment Nahdlatul Ulama University of Surabaya. Also thanks to lectures of the Biochemistry and Molecular Biology Department, Airlangga University had already supported this Depresearartchmen. t, Airlangga University had already supported this research. 6. REFERENCES 6. REFERENCES 1. A. Soni, V.K. Dwivedi, M. Chaudharry, S.M. Shrivastava and V. Naithani. Efficacy of ampucare: A novel herbal formulation for burn wound healing versus other burn medicines. Asian Journal of Biological oSthciener cebusr3(n 1)m:ed18ici–27 (nes. 201A0)sia.n Journal of Biological Sciences 3(1): 18–27 (2010). T.S.N. Hidayat. Peran Topikal Ekstrak Gel Aloe Vera pada Penyembuhan Luka Bakar Derajat Dalam pada pada Penyembuhan Luka Bakar Derajat Dalam pada Funding Information: Partially of research publication was supported by the Institute of Research and the Community Empowerment Nahdlatul Ulama University of Surabaya. Also thanks to lectures of the Biochemistry and Molecular Biology Department, Airlangga University had already supported this research. Publisher Copyright: {\textcopyright} Pakistan Academy of Sciences.",
year = "2021",
language = "English",
volume = "57",
pages = "35--40",
journal = "Proceedings of the Pakistan Academy of Sciences: Part B",
issn = "2518-4261",
publisher = "Pakistan Academy of Sciences",
number = "3",
}