In vitro cytotoxicity study of platelet rich fibrin (PRF) membranes in mesenchymal stem cells (SMSCS) derived from the skin of New Zealand white rabbits

Igo Syaiful Ihsan, Qorri Aynay, Deya Karsari, Aristika Dinaryanti, Nora Ertanti, Helen Susilowati, Purwati, Fedik Abdul Rantam

Research output: Contribution to journalArticlepeer-review

Abstract

Platelet-rich fibrin (PRF) is included in the new generation of autologous platelet concentrations obtained through simple preparation. This material is extremely accessible as it only needs centrifugation to process and does not require the handling of blood, anticoagulants or bovine thrombin.Prior to clinical use, the cytotoxicity of PRF should be assessed to determine the toxic potential of the material. MTT assay is one of the fastest conventional methods used to determined cytotoxicity. This study showed that the membranes of PRF are not toxic, as evidenced by the high percentage of live cells remaining following exposure, 108% for 24 hours and 99.8% for 48 hours. Furthermore, ANOVA analysis showed no significant difference in viability between variable control cells and PRF membrane-exposed cells.

Original languageEnglish
Pages (from-to)4771-4775
Number of pages5
JournalBiochemical and Cellular Archives
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Cytotoxicity
  • PRF membrane
  • SMSCs

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