TY - JOUR
T1 - In vitro cytotoxicity of dragon fruit extract (Hylocereus polyrhizus) on fibroblast cell culture
AU - Juliastuti, Wisnu Setyari
AU - Budi, Hendrik Setia
AU - Karimah, Dinda Akhlakul
N1 - Publisher Copyright:
© 2020, Advanced Scientific Research. All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - The wound from tooth extraction must be handled as soon as possible to prevent any complication, such as: overwhelming pain, bleeding, inflammation, disturbance on mastication function and speech function and causing dangerous infection; to prevent those complications, a medication is administered to help accelerate healing process. These days, there are the development of the usage of natural ingredient as the alternative medication, one of those medications is dragonfruit rind (Hylocereus polyrhizus), because the dragonfruit rind has many benefits, such as antioxidant and antibacterial. But the safety of the dragonfruit rind as alternative medication must be proven through cytotoxicity test by using fibroblast cell culture of Baby Hamster Kidney21 (BHK-21). The aims of this study to determine the toxicity of the extract of the dragonfruit rind (Hylocereus polyrhizus) towards fibroblast cell. The study is laboratory experiment by measuring the level of optical density of fibroblast cell using Methyltiazolyldiphenyl-tetrazolium bromide (MTT) assay. The fibroblast cell culture of BHK-21/C13 is treated with the extract of the dragonfruit rind with the concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. The bigger the percentage of the extract of the dragonfruit rind, the level of optical density becomes smaller. It shows that the higher the concentration of the medication, the smaller the number of cells that survive and 30% concentration is the optimal concentration to have ≥60% of surviving cells. The extract of the dragonfruit rind (Hylocereus polyrhizus) shows toxic effect towards fibroblast cell of BHK-21 from 60% concentration.
AB - The wound from tooth extraction must be handled as soon as possible to prevent any complication, such as: overwhelming pain, bleeding, inflammation, disturbance on mastication function and speech function and causing dangerous infection; to prevent those complications, a medication is administered to help accelerate healing process. These days, there are the development of the usage of natural ingredient as the alternative medication, one of those medications is dragonfruit rind (Hylocereus polyrhizus), because the dragonfruit rind has many benefits, such as antioxidant and antibacterial. But the safety of the dragonfruit rind as alternative medication must be proven through cytotoxicity test by using fibroblast cell culture of Baby Hamster Kidney21 (BHK-21). The aims of this study to determine the toxicity of the extract of the dragonfruit rind (Hylocereus polyrhizus) towards fibroblast cell. The study is laboratory experiment by measuring the level of optical density of fibroblast cell using Methyltiazolyldiphenyl-tetrazolium bromide (MTT) assay. The fibroblast cell culture of BHK-21/C13 is treated with the extract of the dragonfruit rind with the concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. The bigger the percentage of the extract of the dragonfruit rind, the level of optical density becomes smaller. It shows that the higher the concentration of the medication, the smaller the number of cells that survive and 30% concentration is the optimal concentration to have ≥60% of surviving cells. The extract of the dragonfruit rind (Hylocereus polyrhizus) shows toxic effect towards fibroblast cell of BHK-21 from 60% concentration.
KW - Fibroblast cell of BHK-21
KW - MTT assay
KW - The dragonfruit rind (Hylocereus polyrhizus)
UR - http://www.scopus.com/inward/record.url?scp=85089716206&partnerID=8YFLogxK
U2 - 10.31838/ijpr/2020.12.04.195
DO - 10.31838/ijpr/2020.12.04.195
M3 - Article
AN - SCOPUS:85089716206
SN - 0975-2366
VL - 12
SP - 1395
EP - 1400
JO - International Journal of Pharmaceutical Research
JF - International Journal of Pharmaceutical Research
IS - 4
ER -