TY - JOUR
T1 - In vitro antiviral activity of pinus merkusii (Pinaceae) stem bark and cone against dengue virus type-2 (denv-2)
AU - Ansori, Arif Nur Muhammad
AU - Fadholly, Amaq
AU - Proboningrat, Annise
AU - Hayaza, Suhailah
AU - Susilo, Raden Joko Kuncoroningrat
AU - Naw, Sin War
AU - Posa, Gabrielle Ann Villar
AU - Yusrizal, Yuhanis Firza
AU - Sibero, Mada Triandala
AU - Sucipto, Teguh Hari
AU - Soegijanto, Soegeng
N1 - Funding Information:
This study was supported by the Mandat Research Grant Universitas Airlangga (HRMUA) 2019, Institute of Tropical Disease (ITD) the Center of Excellence (COE) program by the Ministry of Research and Technology (KEMENRISTEK) of the Republic of Indonesia, and PMDSU Scholarship - Batch III which was awarded to Arif Nur Muhammad Ansori, Amaq Fadholly, Annise Proboningrat, Suhailah Hayaza, and Raden Joko Kuncoroningrat Susilo. We thank EJA Translation for editing the manuscript.
Funding Information:
This study was supported by the Mandat Research Grant Universitas Airlangga (HRMUA) 2019, Institute of Tropical Disease (ITD) the Center of Excellence (COE) program by the Ministry of Research and Technology (KEMENRISTEK) of the Republic of Indonesia, and PMDSU Scholarship-Batch III which was awarded to Arif Nur Muhammad Ansori, Amaq Fadholly, Annise Proboningrat, Suhailah Hayaza, and Raden Joko Kuncoroningrat Susilo. We thank EJA Translation for editing the manuscript.
Publisher Copyright:
© RJPT All right reserved.
PY - 2021/7/19
Y1 - 2021/7/19
N2 - Dengue is a major mosquito-borne disease that currently has no effective antiviral or vaccine available. Recently, Indonesia is one of the largest countries in the dengue-endemic region, with a total population of more than 250 million. In the present study, the antiviral activity of P. merkusii stem bark and cone were evaluated against dengue virus type-2 (DENV-2; NCBI accession number: KT012509) isolated from Surabaya, Indonesia. We revealed that P. merkusii stem bark and cone inhibited DENV-2 in Vero cells (originally from African green monkey kidney) with IC50= 140.63 μg/mL and 73.78 μg/mL, CC50= 89.65 μg/mL and 249.5 μg/mL, SI= 0.64 and 3.38, respectively. The findings presented here suggest that P. merkusii stem bark and cone exerts potent antiviral activity against DENV-2. Hence, P. merkusii stem bark and cone are potent to inhibit DENV-2 and should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV-2.
AB - Dengue is a major mosquito-borne disease that currently has no effective antiviral or vaccine available. Recently, Indonesia is one of the largest countries in the dengue-endemic region, with a total population of more than 250 million. In the present study, the antiviral activity of P. merkusii stem bark and cone were evaluated against dengue virus type-2 (DENV-2; NCBI accession number: KT012509) isolated from Surabaya, Indonesia. We revealed that P. merkusii stem bark and cone inhibited DENV-2 in Vero cells (originally from African green monkey kidney) with IC50= 140.63 μg/mL and 73.78 μg/mL, CC50= 89.65 μg/mL and 249.5 μg/mL, SI= 0.64 and 3.38, respectively. The findings presented here suggest that P. merkusii stem bark and cone exerts potent antiviral activity against DENV-2. Hence, P. merkusii stem bark and cone are potent to inhibit DENV-2 and should be considered for in vivo evaluation in the development of an effective antiviral compound against DENV-2.
KW - Antiviral
KW - Dengue
KW - Pinus merkusii
KW - Vero Cell
UR - http://www.scopus.com/inward/record.url?scp=85111440519&partnerID=8YFLogxK
U2 - 10.52711/0974-360X.2021.00641
DO - 10.52711/0974-360X.2021.00641
M3 - Article
AN - SCOPUS:85111440519
SN - 0974-3618
VL - 14
SP - 3706
EP - 3709
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 7
ER -
