TY - JOUR
T1 - In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells
AU - Fadholly, Amaq
AU - Proboningrat, Annise
AU - Dewi Iskandar, Regina
AU - Rantam, Fedik
AU - Sudjarwo, Sri
N1 - Publisher Copyright:
© 2019 Journal of Advanced Pharmaceutical Technology & Research Published by Wolters Kluwer - Medknow.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - This study aimed to prepare Annona squamosa leaf extract-loaded chitosan nanoparticles (nano-ASLE) against human colon cancer (WiDr) cells. Nano-ASLE was made with ionic gelation method. Four concentrations of the nano-ASLE (50, 100, 200, and 400 μg/mL) in dimethyl sulfoxide were prepared on WiDr cells to determine the IC50 value using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, it was divided into three groups of concentration of IC50, 2IC50, and 4IC50 and continued with analysis of caspase-3 expression and cell cycle arrest. The results of particles size were obtained 535.1 nm and showed potent cytotoxicity with IC50 292.39 μg/mL. The expression of caspase-3 increased significantly and caused cell cycle arrest at the G2/M phase and induced apoptosis on WiDr cells. Further studies are needed to obtain the loading efficiency, release of drug concentration, and in vivo study of nano-ASLE to suppress WiDr cells.
AB - This study aimed to prepare Annona squamosa leaf extract-loaded chitosan nanoparticles (nano-ASLE) against human colon cancer (WiDr) cells. Nano-ASLE was made with ionic gelation method. Four concentrations of the nano-ASLE (50, 100, 200, and 400 μg/mL) in dimethyl sulfoxide were prepared on WiDr cells to determine the IC50 value using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, it was divided into three groups of concentration of IC50, 2IC50, and 4IC50 and continued with analysis of caspase-3 expression and cell cycle arrest. The results of particles size were obtained 535.1 nm and showed potent cytotoxicity with IC50 292.39 μg/mL. The expression of caspase-3 increased significantly and caused cell cycle arrest at the G2/M phase and induced apoptosis on WiDr cells. Further studies are needed to obtain the loading efficiency, release of drug concentration, and in vivo study of nano-ASLE to suppress WiDr cells.
KW - Annona squamosa
KW - apoptosis
KW - cell cycle
KW - cytotoxicity
UR - http://www.scopus.com/inward/record.url?scp=85073263981&partnerID=8YFLogxK
U2 - 10.4103/japtr.JAPTR_10_19
DO - 10.4103/japtr.JAPTR_10_19
M3 - Article
AN - SCOPUS:85073263981
SN - 2231-4040
VL - 10
SP - 149
EP - 154
JO - Journal of Advanced Pharmaceutical Technology and Research
JF - Journal of Advanced Pharmaceutical Technology and Research
IS - 4
ER -
