In vitro and in silico analysis of phytochemical compounds of 96% ethanol extract of semanggi (Marsilea crenata Presl.) leaves as a bone formation agent

Agnis P.R. Aditama, Burhan Ma'Arif, Hening Laswati, Mangestuti Agil

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Osteoporosis is the result of an imbalance in the rate of bone resorption and bone formation due to a decrease in estrogen. Phytoestrogens are plant compounds with structures and functions similar to estrogen. Phytoestrogens that bind to estrogen receptors in bone cells are able to modulate bone formation. Semanggi (Marsilea crenata Presl.) is a plant that contains phytoestrogens. The purpose of this study was to observe the expression of osteocalcin and predict the content of extract phytoestrogens through a computer simulation study to study the bone formation activity of the 96% ethanol extract of M. crenata leaves on hFOB 1.19 cells. hFOB 1.19 cells were cultured in 24-well microplates, and 96% ethanol extract of M. crenata Presl. leaves was added at 62.5, 125 and 250 ppm. The expression of osteocalcin was analyzed using CLSM immunocytochemistry. Using PyRx 0.8 software and 1ERE protein for molecular docking, the compound was analyzed by computer. The 96% ethanol extract of M. crenata Presl. leaves can increase the expression of osteocalcin, the optimal dose is 125 ppm, and p<0.05 is 881.658 AU. In silico study was obtained six compounds that showed similar activity 17β-estradiol as ER-β agonists. The 96% ethanol extract of M. crenata Presl. leaves contain six compounds that are thought to be phytoestrogens and ER-β agonists, and play a role in increasing bone formation activity and have the potential to be used as an oral drug.

Original languageEnglish
Pages (from-to)881-887
Number of pages7
JournalJournal of Basic and Clinical Physiology and Pharmacology
Volume32
Issue number4
DOIs
Publication statusPublished - 1 Jul 2021

Keywords

  • Marsilea crenata Presl
  • bone formation
  • hFOB 1.19 cell
  • osteocalcin
  • phytoestrogens

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