TY - GEN
T1 - In-silico and In-Vitro Antiplatelet Activity from Chloroform Fraction of Carica papaya L. Leaf Extract
AU - Rohmah, Martina Kurnia
AU - Fickri, Djelang Zainuddin
AU - Anwari, Farida
AU - Nurdianto, Arif Rahman
AU - Putra, Galih Satrio
AU - Sulistyowaty, Melanny Ika
N1 - Publisher Copyright:
© 2022 American Institute of Physics Inc.. All rights reserved.
PY - 2022/1/25
Y1 - 2022/1/25
N2 - Ischemia and stroke are the top ten deadliest diseases in the world, where both of these diseases are related to blockage of blood vessels. Clopidogrel as an antiplatelet agent for the therapy for those diseases still has many side effects. The treatment with Carica papaya L. extract containing alkaloid compounds, traditionally has an anti-platelet effect. This research aimed to observe antiplatelet activity of chloroform extract of Carica papaya L. leaves by in-silico study into ADP (Adenosin Diphosphate) receptor and in-vitro study of platelet rich plasma induced by ADP which was observed with spectrophotometer UV/Vis method. Based on the results of in-silico study, alkaloid compounds such as dehydrocarpain I, dehydrocarpain II and emetin have docking score (-121.77 until -142.75 Kcal / mol) had lower binding energy than Clopidogrel (-111.09 Kcal / mol) toward ADP receptor. The result of in-vitro study, found that the addition of 100 ppm of chloroform fraction extract oí Carica papaya L. leaves inhibited platelet aggregation about 2.55% ± 0.36, which was better than the standard drug, Clopidogrel (2.13% ± 0.34). Based on the results of in-silico and in-vitro study, the chloroform fraction extract of Carica papaya L. which contained alkaloid compounds, had activity to inhibit platelet aggregation, therefore they are potential to be developed as new drugs.
AB - Ischemia and stroke are the top ten deadliest diseases in the world, where both of these diseases are related to blockage of blood vessels. Clopidogrel as an antiplatelet agent for the therapy for those diseases still has many side effects. The treatment with Carica papaya L. extract containing alkaloid compounds, traditionally has an anti-platelet effect. This research aimed to observe antiplatelet activity of chloroform extract of Carica papaya L. leaves by in-silico study into ADP (Adenosin Diphosphate) receptor and in-vitro study of platelet rich plasma induced by ADP which was observed with spectrophotometer UV/Vis method. Based on the results of in-silico study, alkaloid compounds such as dehydrocarpain I, dehydrocarpain II and emetin have docking score (-121.77 until -142.75 Kcal / mol) had lower binding energy than Clopidogrel (-111.09 Kcal / mol) toward ADP receptor. The result of in-vitro study, found that the addition of 100 ppm of chloroform fraction extract oí Carica papaya L. leaves inhibited platelet aggregation about 2.55% ± 0.36, which was better than the standard drug, Clopidogrel (2.13% ± 0.34). Based on the results of in-silico and in-vitro study, the chloroform fraction extract of Carica papaya L. which contained alkaloid compounds, had activity to inhibit platelet aggregation, therefore they are potential to be developed as new drugs.
UR - http://www.scopus.com/inward/record.url?scp=85147259682&partnerID=8YFLogxK
U2 - 10.1063/5.0103700
DO - 10.1063/5.0103700
M3 - Conference contribution
AN - SCOPUS:85147259682
T3 - AIP Conference Proceedings
BT - 8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
A2 - Wibowo, Anjar Tri
A2 - Mardianto, M. Fariz Fadillah
A2 - Rulaningtyas, Riries
A2 - Sakti, Satya Candra Wibawa
A2 - Imron, Muhammad Fauzul
A2 - Ramadhan, Rico
PB - American Institute of Physics Inc.
T2 - 8th International Conference and Workshop on Basic and Applied Science, ICOWOBAS 2021
Y2 - 25 August 2021 through 26 August 2021
ER -