TY - JOUR
T1 - Implantation of platelet rich fibrin and allogenic mesenchymal stem cells facilitate the healing of muscle injury
T2 - An experimental study on animal
AU - Utomo, Dwikora N.
AU - Mahyudin, Ferdiansyah
AU - Hernugrahanto, Kukuh D.
AU - Suroto, Heri
AU - Chilmi, Muhammad Zaim
AU - Rantam, Fedik Abdul
N1 - Publisher Copyright:
© 2018 The Authors
PY - 2018
Y1 - 2018
N2 - Introduction: Muscle injury has caused adverse impacts on athletes' performance. Muscle injury treatments are based on the degree of severity. Unfortunately, in extensive injuries, surgical treatments are often unsatisfactory especially in athletes with high functional demand. More effort is needed to achieve a better result in muscle injury healing. The use of platelet-rich fibrin (PRF) and mesenchymal stem cell (MSC) would provide all the necessary factors to achieve good tissue healing: cells, growth factors, and scaffold. The study aims to evaluate the role of PRF and MSC in facilitating the healing of muscle injury on animal models. Methods: A model defect was created in the gastrocnemius muscle of each hind leg of twenty New Zealand white rabbits. All legs were randomly divided into four groups: (1) control; (2) PRF-only; (3) MSC-only, and (4) PRF-and-MSC group. After two and four weeks, the muscle was retrieved and sent for immunohistochemistry examination to evaluate the expression of Pax7 and MyoD protein. Results: The mean score of all treated group was higher compared to the control group. The group that received both PRF and MSC showed the highest score. Conclusion: Considering the promising result, application of PRF and MSC could be an option for the treatment of muscle injury as this would provide all necessary elements of tissue engineering to facilitate the healing process of muscle: the cells, the scaffold, and the growth factors.
AB - Introduction: Muscle injury has caused adverse impacts on athletes' performance. Muscle injury treatments are based on the degree of severity. Unfortunately, in extensive injuries, surgical treatments are often unsatisfactory especially in athletes with high functional demand. More effort is needed to achieve a better result in muscle injury healing. The use of platelet-rich fibrin (PRF) and mesenchymal stem cell (MSC) would provide all the necessary factors to achieve good tissue healing: cells, growth factors, and scaffold. The study aims to evaluate the role of PRF and MSC in facilitating the healing of muscle injury on animal models. Methods: A model defect was created in the gastrocnemius muscle of each hind leg of twenty New Zealand white rabbits. All legs were randomly divided into four groups: (1) control; (2) PRF-only; (3) MSC-only, and (4) PRF-and-MSC group. After two and four weeks, the muscle was retrieved and sent for immunohistochemistry examination to evaluate the expression of Pax7 and MyoD protein. Results: The mean score of all treated group was higher compared to the control group. The group that received both PRF and MSC showed the highest score. Conclusion: Considering the promising result, application of PRF and MSC could be an option for the treatment of muscle injury as this would provide all necessary elements of tissue engineering to facilitate the healing process of muscle: the cells, the scaffold, and the growth factors.
KW - Allogenic
KW - Mesenchymal stem cell
KW - Muscle injury
KW - Platelet-rich fibrin
UR - http://www.scopus.com/inward/record.url?scp=85044937876&partnerID=8YFLogxK
U2 - 10.1016/j.ijso.2018.03.001
DO - 10.1016/j.ijso.2018.03.001
M3 - Article
AN - SCOPUS:85044937876
SN - 2405-8572
VL - 11
SP - 4
EP - 9
JO - International Journal of Surgery Open
JF - International Journal of Surgery Open
ER -