TY - JOUR
T1 - Impact of the use of ethanolic extract of propolis, flavonoid and non-flavonoid propolis for direct pulp capping in collagen type i density
AU - Sabir, Ardo
AU - Mooduto, Latief
AU - Kaelan, Cahyono
AU - Horax, Sherly
PY - 2016
Y1 - 2016
N2 - Aim: To analysis collagen type I density on inflamed rat dental pulp after capping with propolis. Methods: Flavonoid and non-flavonoid substances were purified from propolis. Eighty male rats were divided into five groups, each group consisting of 16 rats. As a negative control (group I), rats were not conducted any treatment. A class I cavity was prepared on the occlusal surface of right maxillary first molar. Dental pulp was exposed and allowed in oral environment for 60 minutes, then dental pulp capping with ethanolic extract of propolis (group II), flavonoid propolis (group III), non-flavonoid propolis (group IV), or calcium hydroxide as positive control (group V). Rats were sacrificed at 6 hours, 2, 4 or 7 days, biopsy samples were obtained, stained and viewed by light microscope. Data was statistically analysis using Friedman and Kruskal-Wallis tests. Results: Except in group I, collagen type I density was increased in group II, III, and V with the longer of observation time periods. However, in group IV, collagen type I density increased only on day 7. No statistically significant differences of collagen type I density among the groups for each time period were found. Conclusions: Propolis and flavonoid propolis may increase collagen density on inflamed rat dental pulp.
AB - Aim: To analysis collagen type I density on inflamed rat dental pulp after capping with propolis. Methods: Flavonoid and non-flavonoid substances were purified from propolis. Eighty male rats were divided into five groups, each group consisting of 16 rats. As a negative control (group I), rats were not conducted any treatment. A class I cavity was prepared on the occlusal surface of right maxillary first molar. Dental pulp was exposed and allowed in oral environment for 60 minutes, then dental pulp capping with ethanolic extract of propolis (group II), flavonoid propolis (group III), non-flavonoid propolis (group IV), or calcium hydroxide as positive control (group V). Rats were sacrificed at 6 hours, 2, 4 or 7 days, biopsy samples were obtained, stained and viewed by light microscope. Data was statistically analysis using Friedman and Kruskal-Wallis tests. Results: Except in group I, collagen type I density was increased in group II, III, and V with the longer of observation time periods. However, in group IV, collagen type I density increased only on day 7. No statistically significant differences of collagen type I density among the groups for each time period were found. Conclusions: Propolis and flavonoid propolis may increase collagen density on inflamed rat dental pulp.
KW - Collagen type I
KW - Dental pulp
KW - Inflammation
KW - Propolis
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=85030183225&partnerID=8YFLogxK
U2 - 10.20396/bjos.v15i4.8650037
DO - 10.20396/bjos.v15i4.8650037
M3 - Article
AN - SCOPUS:85030183225
SN - 1677-3217
VL - 15
SP - 264
EP - 268
JO - Brazilian Journal of Oral Sciences
JF - Brazilian Journal of Oral Sciences
IS - 4
ER -